Experiment: KLEB24 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17515 and BWI76_RS17520 are separated by 12 nucleotides BWI76_RS17520 and BWI76_RS17525 are separated by 48 nucleotides BWI76_RS17525 and BWI76_RS17530 overlap by 96 nucleotides
BWI76_RS17515: BWI76_RS17515 - dihydropyrimidinase, at 3,632,423 to 3,633,829
_RS17515
BWI76_RS17520: BWI76_RS17520 - carbamate kinase, at 3,633,842 to 3,634,774
_RS17520
BWI76_RS17525: BWI76_RS17525 - XshC-Cox1-family protein, at 3,634,823 to 3,635,656
_RS17525
BWI76_RS17530: BWI76_RS17530 - hypothetical protein, at 3,635,561 to 3,635,938
_RS17530
Position (kb)
3633
3634
3635 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3632.876 kb on - strand, within BWI76_RS17515 at 3633.047 kb on - strand, within BWI76_RS17515 at 3633.173 kb on - strand at 3633.284 kb on + strand, within BWI76_RS17515 at 3633.354 kb on + strand, within BWI76_RS17515 at 3633.904 kb on + strand at 3633.904 kb on + strand at 3634.180 kb on - strand, within BWI76_RS17520 at 3634.396 kb on - strand, within BWI76_RS17520 at 3634.433 kb on + strand, within BWI76_RS17520 at 3634.472 kb on + strand, within BWI76_RS17520 at 3634.782 kb on - strand at 3635.433 kb on - strand, within BWI76_RS17525 at 3635.549 kb on + strand, within BWI76_RS17525 at 3635.563 kb on + strand at 3635.571 kb on - strand at 3635.582 kb on + strand at 3635.590 kb on - strand at 3635.603 kb on + strand at 3635.613 kb on - strand at 3635.615 kb on + strand at 3635.623 kb on - strand at 3635.623 kb on - strand at 3635.623 kb on - strand at 3635.623 kb on - strand at 3635.631 kb on - strand at 3635.670 kb on - strand, within BWI76_RS17530 at 3635.671 kb on - strand, within BWI76_RS17530 at 3635.685 kb on + strand, within BWI76_RS17530 at 3635.765 kb on + strand, within BWI76_RS17530
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB24 MOI 1 remove 3,632,876 - BWI76_RS17515 0.32 -1.1 3,633,047 - BWI76_RS17515 0.44 -1.1 3,633,173 - +0.5 3,633,284 + BWI76_RS17515 0.61 -1.1 3,633,354 + BWI76_RS17515 0.66 -0.1 3,633,904 + +0.2 3,633,904 + +0.7 3,634,180 - BWI76_RS17520 0.36 -0.5 3,634,396 - BWI76_RS17520 0.59 -1.6 3,634,433 + BWI76_RS17520 0.63 -0.2 3,634,472 + BWI76_RS17520 0.68 -0.4 3,634,782 - +0.1 3,635,433 - BWI76_RS17525 0.73 +0.7 3,635,549 + BWI76_RS17525 0.87 -0.7 3,635,563 + -0.1 3,635,571 - -0.3 3,635,582 + +0.2 3,635,590 - -0.5 3,635,603 + -0.7 3,635,613 - -0.2 3,635,615 + +4.3 3,635,623 - +1.5 3,635,623 - +0.3 3,635,623 - -2.3 3,635,623 - -0.0 3,635,631 - +0.9 3,635,670 - BWI76_RS17530 0.29 +0.7 3,635,671 - BWI76_RS17530 0.29 -1.1 3,635,685 + BWI76_RS17530 0.33 -0.5 3,635,765 + BWI76_RS17530 0.54 +2.1
Or see this region's nucleotide sequence