Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16820

Experiment: KLEB24 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16810 and BWI76_RS16815 overlap by 4 nucleotidesBWI76_RS16815 and BWI76_RS16820 are separated by 16 nucleotidesBWI76_RS16820 and BWI76_RS16825 overlap by 4 nucleotides BWI76_RS16810: BWI76_RS16810 - RpiR family transcriptional regulator, at 3,485,048 to 3,485,947 _RS16810 BWI76_RS16815: BWI76_RS16815 - periplasmic binding protein, at 3,485,944 to 3,486,891 _RS16815 BWI76_RS16820: BWI76_RS16820 - iron ABC transporter, at 3,486,908 to 3,487,960 _RS16820 BWI76_RS16825: BWI76_RS16825 - heme ABC transporter, at 3,487,957 to 3,488,982 _RS16825 Position (kb) 3486 3487 3488Strain fitness (log2 ratio) -3 -2 -1 0 1at 3486.017 kb on + strandat 3486.017 kb on + strandat 3486.032 kb on - strandat 3486.033 kb on - strandat 3486.588 kb on - strand, within BWI76_RS16815at 3486.654 kb on + strand, within BWI76_RS16815at 3487.049 kb on + strand, within BWI76_RS16820at 3487.075 kb on + strand, within BWI76_RS16820at 3487.353 kb on - strand, within BWI76_RS16820at 3487.378 kb on + strand, within BWI76_RS16820at 3487.656 kb on - strand, within BWI76_RS16820at 3487.656 kb on - strand, within BWI76_RS16820at 3487.738 kb on + strand, within BWI76_RS16820at 3488.148 kb on + strand, within BWI76_RS16825at 3488.253 kb on - strand, within BWI76_RS16825at 3488.499 kb on + strand, within BWI76_RS16825at 3488.599 kb on + strand, within BWI76_RS16825at 3488.607 kb on - strand, within BWI76_RS16825at 3488.667 kb on + strand, within BWI76_RS16825

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB24 MOI 1
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3,486,017 + +0.3
3,486,017 + +1.1
3,486,032 - +0.2
3,486,033 - +0.3
3,486,588 - BWI76_RS16815 0.68 -3.2
3,486,654 + BWI76_RS16815 0.75 -0.5
3,487,049 + BWI76_RS16820 0.13 -1.4
3,487,075 + BWI76_RS16820 0.16 +0.1
3,487,353 - BWI76_RS16820 0.42 -0.7
3,487,378 + BWI76_RS16820 0.45 -2.3
3,487,656 - BWI76_RS16820 0.71 -1.8
3,487,656 - BWI76_RS16820 0.71 -0.1
3,487,738 + BWI76_RS16820 0.79 +0.1
3,488,148 + BWI76_RS16825 0.19 -1.2
3,488,253 - BWI76_RS16825 0.29 -0.2
3,488,499 + BWI76_RS16825 0.53 +0.6
3,488,599 + BWI76_RS16825 0.63 -1.1
3,488,607 - BWI76_RS16825 0.63 -0.0
3,488,667 + BWI76_RS16825 0.69 -0.8

Or see this region's nucleotide sequence