Experiment: KLEB24 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16085 and BWI76_RS16090 overlap by 4 nucleotides BWI76_RS16090 and BWI76_RS16095 overlap by 20 nucleotides BWI76_RS16095 and BWI76_RS16100 are separated by 67 nucleotides
BWI76_RS16085: BWI76_RS16085 - two-component system sensor histidine kinase DcuS, at 3,335,222 to 3,336,841
_RS16085
BWI76_RS16090: BWI76_RS16090 - two-component system response regulator DcuR, at 3,336,838 to 3,337,557
_RS16090
BWI76_RS16095: BWI76_RS16095 - thiamine biosynthesis protein ApbE, at 3,337,538 to 3,338,488
_RS16095
BWI76_RS16100: BWI76_RS16100 - flavocytochrome c, at 3,338,556 to 3,341,333
_RS16100
Position (kb)
3336
3337
3338 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3335.919 kb on + strand, within BWI76_RS16085 at 3335.981 kb on - strand, within BWI76_RS16085 at 3336.249 kb on - strand, within BWI76_RS16085 at 3336.323 kb on - strand, within BWI76_RS16085 at 3336.444 kb on + strand, within BWI76_RS16085 at 3336.452 kb on - strand, within BWI76_RS16085 at 3336.568 kb on + strand, within BWI76_RS16085 at 3336.650 kb on + strand, within BWI76_RS16085 at 3336.822 kb on + strand at 3337.028 kb on - strand, within BWI76_RS16090 at 3337.119 kb on - strand, within BWI76_RS16090 at 3337.143 kb on - strand, within BWI76_RS16090 at 3337.825 kb on + strand, within BWI76_RS16095 at 3338.170 kb on + strand, within BWI76_RS16095 at 3338.331 kb on + strand, within BWI76_RS16095 at 3338.426 kb on - strand at 3338.468 kb on - strand at 3338.505 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB24 MOI 1 remove 3,335,919 + BWI76_RS16085 0.43 +1.4 3,335,981 - BWI76_RS16085 0.47 -0.1 3,336,249 - BWI76_RS16085 0.63 +0.9 3,336,323 - BWI76_RS16085 0.68 -0.9 3,336,444 + BWI76_RS16085 0.75 +0.3 3,336,452 - BWI76_RS16085 0.76 +2.4 3,336,568 + BWI76_RS16085 0.83 -2.2 3,336,650 + BWI76_RS16085 0.88 -1.9 3,336,822 + +1.0 3,337,028 - BWI76_RS16090 0.26 +0.2 3,337,119 - BWI76_RS16090 0.39 -0.4 3,337,143 - BWI76_RS16090 0.42 -0.9 3,337,825 + BWI76_RS16095 0.30 -3.3 3,338,170 + BWI76_RS16095 0.66 +0.9 3,338,331 + BWI76_RS16095 0.83 -0.2 3,338,426 - +1.3 3,338,468 - -0.4 3,338,505 - +0.8
Or see this region's nucleotide sequence