Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS13995

Experiment: KLEB24 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS13990 and BWI76_RS13995 are separated by 184 nucleotidesBWI76_RS13995 and BWI76_RS14000 are separated by 103 nucleotidesBWI76_RS14000 and BWI76_RS14005 are separated by 2 nucleotides BWI76_RS13990: BWI76_RS13990 - allophanate hydrolase, at 2,921,893 to 2,923,689 _RS13990 BWI76_RS13995: BWI76_RS13995 - penicillin-binding protein 2, at 2,923,874 to 2,925,793 _RS13995 BWI76_RS14000: BWI76_RS14000 - glutamine amidotransferase, at 2,925,897 to 2,926,640 _RS14000 BWI76_RS14005: BWI76_RS14005 - TetR family transcriptional regulator, at 2,926,643 to 2,927,191 _RS14005 Position (kb) 2923 2924 2925 2926Strain fitness (log2 ratio) -1 0 1at 2922.966 kb on + strand, within BWI76_RS13990at 2923.183 kb on - strand, within BWI76_RS13990at 2923.518 kb on - strandat 2924.282 kb on - strand, within BWI76_RS13995at 2924.454 kb on - strand, within BWI76_RS13995at 2924.576 kb on + strand, within BWI76_RS13995at 2924.944 kb on + strand, within BWI76_RS13995at 2925.323 kb on - strand, within BWI76_RS13995at 2925.419 kb on - strand, within BWI76_RS13995at 2925.519 kb on - strand, within BWI76_RS13995at 2926.067 kb on + strand, within BWI76_RS14000at 2926.339 kb on - strand, within BWI76_RS14000at 2926.343 kb on + strand, within BWI76_RS14000at 2926.523 kb on - strand, within BWI76_RS14000at 2926.570 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB24 MOI 1
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2,922,966 + BWI76_RS13990 0.60 -0.1
2,923,183 - BWI76_RS13990 0.72 +0.2
2,923,518 - -1.4
2,924,282 - BWI76_RS13995 0.21 -0.1
2,924,454 - BWI76_RS13995 0.30 -0.9
2,924,576 + BWI76_RS13995 0.37 -1.2
2,924,944 + BWI76_RS13995 0.56 -1.2
2,925,323 - BWI76_RS13995 0.75 +0.1
2,925,419 - BWI76_RS13995 0.80 -0.4
2,925,519 - BWI76_RS13995 0.86 -1.2
2,926,067 + BWI76_RS14000 0.23 -1.4
2,926,339 - BWI76_RS14000 0.59 +0.4
2,926,343 + BWI76_RS14000 0.60 +0.0
2,926,523 - BWI76_RS14000 0.84 +0.3
2,926,570 + +0.8

Or see this region's nucleotide sequence