Experiment: KLEB24 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13960 and BWI76_RS13965 are separated by 24 nucleotides BWI76_RS13965 and BWI76_RS13970 overlap by 4 nucleotides BWI76_RS13970 and BWI76_RS13975 are separated by 4 nucleotides BWI76_RS13975 and BWI76_RS13980 are separated by 10 nucleotides
BWI76_RS13960: BWI76_RS13960 - urea ABC transporter periplasmic protein, at 2,914,072 to 2,915,130
_RS13960
BWI76_RS13965: BWI76_RS13965 - binding-protein-dependent transport system inner membrane protein, at 2,915,155 to 2,915,970
_RS13965
BWI76_RS13970: BWI76_RS13970 - ABC transporter, at 2,915,967 to 2,916,743
_RS13970
BWI76_RS13975: BWI76_RS13975 - urea carboxylase, at 2,916,748 to 2,917,476
_RS13975
BWI76_RS13980: BWI76_RS13980 - hypothetical protein, at 2,917,487 to 2,918,122
_RS13980
Position (kb) 2915
2916
2917 Strain fitness (log2 ratio) -2
-1
0
1 at 2915.563 kb on - strand, within BWI76_RS13965 at 2915.752 kb on - strand, within BWI76_RS13965 at 2916.018 kb on + strand at 2916.026 kb on - strand at 2916.576 kb on + strand, within BWI76_RS13970 at 2916.777 kb on - strand at 2916.778 kb on - strand at 2917.061 kb on - strand, within BWI76_RS13975 at 2917.396 kb on + strand, within BWI76_RS13975
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB24 MOI 1 remove 2,915,563 - BWI76_RS13965 0.50 +0.1 2,915,752 - BWI76_RS13965 0.73 +0.1 2,916,018 + +0.8 2,916,026 - -0.5 2,916,576 + BWI76_RS13970 0.78 -1.7 2,916,777 - +0.6 2,916,778 - -0.7 2,917,061 - BWI76_RS13975 0.43 -2.0 2,917,396 + BWI76_RS13975 0.89 +0.8
Or see this region's nucleotide sequence