Experiment: KLEB24 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10565 and BWI76_RS10570 are separated by 214 nucleotides BWI76_RS10570 and BWI76_RS10575 are separated by 9 nucleotides BWI76_RS10575 and BWI76_RS10580 overlap by 1 nucleotides
BWI76_RS10565: BWI76_RS10565 - sugar isomerase, at 2,231,816 to 2,232,655
_RS10565
BWI76_RS10570: BWI76_RS10570 - cysteine ABC transporter substrate-binding protein, at 2,232,870 to 2,233,655
_RS10570
BWI76_RS10575: BWI76_RS10575 - polar amino acid ABC transporter permease, at 2,233,665 to 2,234,330
_RS10575
BWI76_RS10580: BWI76_RS10580 - amino acid ABC transporter permease, at 2,234,330 to 2,234,986
_RS10580
Position (kb)
2232
2233
2234 Strain fitness (log2 ratio)
-2
-1
0
1 at 2231.926 kb on - strand, within BWI76_RS10565 at 2232.085 kb on - strand, within BWI76_RS10565 at 2232.153 kb on + strand, within BWI76_RS10565 at 2232.155 kb on - strand, within BWI76_RS10565 at 2232.228 kb on - strand, within BWI76_RS10565 at 2232.320 kb on + strand, within BWI76_RS10565 at 2232.893 kb on + strand at 2232.893 kb on + strand at 2233.032 kb on - strand, within BWI76_RS10570 at 2233.157 kb on - strand, within BWI76_RS10570 at 2233.221 kb on - strand, within BWI76_RS10570 at 2233.384 kb on - strand, within BWI76_RS10570 at 2233.713 kb on - strand at 2233.851 kb on - strand, within BWI76_RS10575 at 2233.978 kb on + strand, within BWI76_RS10575 at 2234.275 kb on - strand at 2234.506 kb on + strand, within BWI76_RS10580 at 2234.514 kb on - strand, within BWI76_RS10580
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB24 MOI 1 remove 2,231,926 - BWI76_RS10565 0.13 -1.1 2,232,085 - BWI76_RS10565 0.32 +0.3 2,232,153 + BWI76_RS10565 0.40 -0.6 2,232,155 - BWI76_RS10565 0.40 -0.1 2,232,228 - BWI76_RS10565 0.49 -0.4 2,232,320 + BWI76_RS10565 0.60 +1.4 2,232,893 + -0.2 2,232,893 + +0.2 2,233,032 - BWI76_RS10570 0.21 -0.7 2,233,157 - BWI76_RS10570 0.37 -1.2 2,233,221 - BWI76_RS10570 0.45 -1.3 2,233,384 - BWI76_RS10570 0.65 -0.4 2,233,713 - -0.3 2,233,851 - BWI76_RS10575 0.28 +0.6 2,233,978 + BWI76_RS10575 0.47 -0.2 2,234,275 - -1.9 2,234,506 + BWI76_RS10580 0.27 -0.0 2,234,514 - BWI76_RS10580 0.28 -1.9
Or see this region's nucleotide sequence