Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10055

Experiment: KLEB24 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10045 and BWI76_RS10050 are separated by 190 nucleotidesBWI76_RS10050 and BWI76_RS10055 are separated by 15 nucleotidesBWI76_RS10055 and BWI76_RS10060 are separated by 51 nucleotidesBWI76_RS10060 and BWI76_RS10065 overlap by 4 nucleotidesBWI76_RS10065 and BWI76_RS10070 are separated by 89 nucleotides BWI76_RS10045: BWI76_RS10045 - 23S rRNA (cytosine(1962)-C(5))-methyltransferase, at 2,128,323 to 2,129,525 _RS10045 BWI76_RS10050: BWI76_RS10050 - outer membrane protein, at 2,129,716 to 2,130,270 _RS10050 BWI76_RS10055: BWI76_RS10055 - AraC family transcriptional regulator, at 2,130,286 to 2,131,074 _RS10055 BWI76_RS10060: BWI76_RS10060 - acylphosphatase, at 2,131,126 to 2,131,407 _RS10060 BWI76_RS10065: BWI76_RS10065 - sulfurtransferase TusE, at 2,131,404 to 2,131,733 _RS10065 BWI76_RS10070: BWI76_RS10070 - BAX inhibitor protein, at 2,131,823 to 2,132,482 _RS10070 Position (kb) 2130 2131 2132Strain fitness (log2 ratio) -1 0 1at 2129.803 kb on + strand, within BWI76_RS10050at 2129.811 kb on - strand, within BWI76_RS10050at 2129.980 kb on + strand, within BWI76_RS10050at 2130.069 kb on - strand, within BWI76_RS10050at 2130.140 kb on + strand, within BWI76_RS10050at 2130.164 kb on + strand, within BWI76_RS10050at 2130.246 kb on + strandat 2130.784 kb on + strand, within BWI76_RS10055at 2130.866 kb on + strand, within BWI76_RS10055at 2131.088 kb on + strandat 2131.358 kb on + strand, within BWI76_RS10060

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB24 MOI 1
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2,129,803 + BWI76_RS10050 0.16 -0.5
2,129,811 - BWI76_RS10050 0.17 +0.2
2,129,980 + BWI76_RS10050 0.48 +1.0
2,130,069 - BWI76_RS10050 0.64 +0.4
2,130,140 + BWI76_RS10050 0.76 -0.4
2,130,164 + BWI76_RS10050 0.81 +0.6
2,130,246 + -0.4
2,130,784 + BWI76_RS10055 0.63 -0.8
2,130,866 + BWI76_RS10055 0.74 -0.6
2,131,088 + +1.5
2,131,358 + BWI76_RS10060 0.82 -0.7

Or see this region's nucleotide sequence