Experiment: KLEB24 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09780 and BWI76_RS09785 are separated by 150 nucleotides BWI76_RS09785 and BWI76_RS09790 are separated by 215 nucleotides BWI76_RS09790 and BWI76_RS09795 are separated by 45 nucleotides
BWI76_RS09780: BWI76_RS09780 - 30S ribosomal protein S1, at 2,050,507 to 2,052,180
_RS09780
BWI76_RS09785: BWI76_RS09785 - integration host factor subunit beta, at 2,052,331 to 2,052,618
_RS09785
BWI76_RS09790: BWI76_RS09790 - ComEC family protein, at 2,052,834 to 2,055,089
_RS09790
BWI76_RS09795: BWI76_RS09795 - lipid ABC transporter permease/ATP-binding protein, at 2,055,135 to 2,056,883
_RS09795
Position (kb)
2052
2053
2054
2055
2056 Strain fitness (log2 ratio)
-1
0
1 at 2052.284 kb on + strand at 2052.704 kb on - strand at 2052.887 kb on - strand at 2052.918 kb on + strand at 2052.962 kb on + strand at 2053.199 kb on + strand, within BWI76_RS09790 at 2053.214 kb on + strand, within BWI76_RS09790 at 2053.229 kb on + strand, within BWI76_RS09790 at 2053.264 kb on - strand, within BWI76_RS09790 at 2053.550 kb on - strand, within BWI76_RS09790 at 2053.658 kb on - strand, within BWI76_RS09790 at 2053.658 kb on - strand, within BWI76_RS09790 at 2053.959 kb on - strand, within BWI76_RS09790 at 2054.203 kb on + strand, within BWI76_RS09790 at 2054.360 kb on - strand, within BWI76_RS09790 at 2054.500 kb on + strand, within BWI76_RS09790 at 2054.560 kb on - strand, within BWI76_RS09790 at 2054.668 kb on - strand, within BWI76_RS09790 at 2054.733 kb on - strand, within BWI76_RS09790 at 2054.852 kb on - strand, within BWI76_RS09790 at 2054.858 kb on - strand, within BWI76_RS09790 at 2054.914 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB24 MOI 1 remove 2,052,284 + +0.9 2,052,704 - +0.8 2,052,887 - +0.0 2,052,918 + +0.3 2,052,962 + -0.2 2,053,199 + BWI76_RS09790 0.16 +1.4 2,053,214 + BWI76_RS09790 0.17 +1.4 2,053,229 + BWI76_RS09790 0.18 +0.3 2,053,264 - BWI76_RS09790 0.19 +0.2 2,053,550 - BWI76_RS09790 0.32 +1.5 2,053,658 - BWI76_RS09790 0.37 -0.5 2,053,658 - BWI76_RS09790 0.37 +1.0 2,053,959 - BWI76_RS09790 0.50 +0.8 2,054,203 + BWI76_RS09790 0.61 +0.2 2,054,360 - BWI76_RS09790 0.68 +0.3 2,054,500 + BWI76_RS09790 0.74 +0.0 2,054,560 - BWI76_RS09790 0.77 -0.1 2,054,668 - BWI76_RS09790 0.81 +0.2 2,054,733 - BWI76_RS09790 0.84 +1.1 2,054,852 - BWI76_RS09790 0.89 -0.0 2,054,858 - BWI76_RS09790 0.90 +0.3 2,054,914 - -0.6
Or see this region's nucleotide sequence