Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08960

Experiment: KLEB24 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08955 and BWI76_RS08960 overlap by 6 nucleotidesBWI76_RS08960 and BWI76_RS08965 are separated by 110 nucleotidesBWI76_RS08965 and BWI76_RS08970 overlap by 4 nucleotides BWI76_RS08955: BWI76_RS08955 - 23S rRNA (adenine(1618)-N(6))-methyltransferase, at 1,886,653 to 1,887,567 _RS08955 BWI76_RS08960: BWI76_RS08960 - mechanosensitive channel protein, at 1,887,562 to 1,889,772 _RS08960 BWI76_RS08965: BWI76_RS08965 - glutamine ABC transporter ATP-binding protein, at 1,889,883 to 1,890,605 _RS08965 BWI76_RS08970: BWI76_RS08970 - glutamine ABC transporter permease GlnP, at 1,890,602 to 1,891,261 _RS08970 Position (kb) 1887 1888 1889 1890Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1886.682 kb on + strandat 1886.710 kb on - strandat 1886.838 kb on + strand, within BWI76_RS08955at 1886.856 kb on - strand, within BWI76_RS08955at 1887.157 kb on - strand, within BWI76_RS08955at 1887.196 kb on + strand, within BWI76_RS08955at 1887.209 kb on - strand, within BWI76_RS08955at 1887.274 kb on - strand, within BWI76_RS08955at 1887.274 kb on - strand, within BWI76_RS08955at 1887.274 kb on - strand, within BWI76_RS08955at 1887.446 kb on - strand, within BWI76_RS08955at 1888.058 kb on + strand, within BWI76_RS08960at 1888.343 kb on - strand, within BWI76_RS08960at 1888.343 kb on - strand, within BWI76_RS08960at 1888.640 kb on + strand, within BWI76_RS08960at 1888.749 kb on + strand, within BWI76_RS08960at 1888.839 kb on + strand, within BWI76_RS08960at 1888.896 kb on - strand, within BWI76_RS08960at 1889.471 kb on + strand, within BWI76_RS08960at 1889.479 kb on - strand, within BWI76_RS08960at 1889.803 kb on + strandat 1889.912 kb on + strandat 1890.342 kb on + strand, within BWI76_RS08965at 1890.421 kb on - strand, within BWI76_RS08965at 1890.461 kb on + strand, within BWI76_RS08965at 1890.469 kb on - strand, within BWI76_RS08965at 1890.474 kb on + strand, within BWI76_RS08965at 1890.487 kb on - strand, within BWI76_RS08965at 1890.645 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB24 MOI 1
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1,886,682 + +0.5
1,886,710 - +3.2
1,886,838 + BWI76_RS08955 0.20 +0.7
1,886,856 - BWI76_RS08955 0.22 -0.4
1,887,157 - BWI76_RS08955 0.55 -0.1
1,887,196 + BWI76_RS08955 0.59 -0.9
1,887,209 - BWI76_RS08955 0.61 -0.3
1,887,274 - BWI76_RS08955 0.68 +0.3
1,887,274 - BWI76_RS08955 0.68 -1.5
1,887,274 - BWI76_RS08955 0.68 +1.3
1,887,446 - BWI76_RS08955 0.87 -0.6
1,888,058 + BWI76_RS08960 0.22 -0.7
1,888,343 - BWI76_RS08960 0.35 -0.5
1,888,343 - BWI76_RS08960 0.35 +0.1
1,888,640 + BWI76_RS08960 0.49 +0.5
1,888,749 + BWI76_RS08960 0.54 +0.6
1,888,839 + BWI76_RS08960 0.58 +0.1
1,888,896 - BWI76_RS08960 0.60 -0.8
1,889,471 + BWI76_RS08960 0.86 +0.7
1,889,479 - BWI76_RS08960 0.87 +0.1
1,889,803 + -0.1
1,889,912 + -0.9
1,890,342 + BWI76_RS08965 0.63 -3.0
1,890,421 - BWI76_RS08965 0.74 -2.0
1,890,461 + BWI76_RS08965 0.80 -0.3
1,890,469 - BWI76_RS08965 0.81 +0.4
1,890,474 + BWI76_RS08965 0.82 -0.1
1,890,487 - BWI76_RS08965 0.84 -0.1
1,890,645 - -0.5

Or see this region's nucleotide sequence