Experiment: KLEB24 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02085 and BWI76_RS02090 overlap by 4 nucleotides BWI76_RS02090 and BWI76_RS02095 are separated by 182 nucleotides
BWI76_RS02085: BWI76_RS02085 - cation acetate symporter, at 429,691 to 431,340
_RS02085
BWI76_RS02090: BWI76_RS02090 - membrane protein, at 431,337 to 431,651
_RS02090
BWI76_RS02095: BWI76_RS02095 - acetyl-coenzyme A synthetase, at 431,834 to 433,792
_RS02095
Position (kb) 431
432 Strain fitness (log2 ratio) -2
-1
0
1
2 at 430.405 kb on + strand, within BWI76_RS02085 at 430.481 kb on - strand, within BWI76_RS02085 at 430.525 kb on - strand, within BWI76_RS02085 at 430.538 kb on - strand, within BWI76_RS02085 at 430.673 kb on - strand, within BWI76_RS02085 at 430.696 kb on + strand, within BWI76_RS02085 at 430.745 kb on - strand, within BWI76_RS02085 at 430.888 kb on + strand, within BWI76_RS02085 at 430.914 kb on + strand, within BWI76_RS02085 at 430.922 kb on - strand, within BWI76_RS02085 at 430.976 kb on - strand, within BWI76_RS02085 at 431.056 kb on - strand, within BWI76_RS02085 at 431.056 kb on - strand, within BWI76_RS02085 at 431.082 kb on - strand, within BWI76_RS02085 at 431.192 kb on + strand at 431.200 kb on - strand at 431.265 kb on - strand at 431.265 kb on - strand at 431.270 kb on - strand at 431.323 kb on + strand at 431.344 kb on + strand at 431.502 kb on - strand, within BWI76_RS02090 at 431.536 kb on - strand, within BWI76_RS02090 at 431.559 kb on - strand, within BWI76_RS02090 at 431.668 kb on - strand at 431.726 kb on - strand at 431.728 kb on + strand at 431.762 kb on + strand at 431.770 kb on - strand at 431.815 kb on + strand at 431.847 kb on + strand at 431.901 kb on + strand at 432.063 kb on + strand, within BWI76_RS02095 at 432.066 kb on - strand at 432.071 kb on - strand, within BWI76_RS02095 at 432.094 kb on + strand, within BWI76_RS02095 at 432.094 kb on + strand, within BWI76_RS02095 at 432.110 kb on + strand, within BWI76_RS02095 at 432.126 kb on + strand, within BWI76_RS02095 at 432.297 kb on + strand, within BWI76_RS02095 at 432.381 kb on + strand, within BWI76_RS02095 at 432.457 kb on + strand, within BWI76_RS02095 at 432.526 kb on + strand, within BWI76_RS02095 at 432.620 kb on - strand, within BWI76_RS02095
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB24 MOI 1 remove 430,405 + BWI76_RS02085 0.43 -1.9 430,481 - BWI76_RS02085 0.48 -0.9 430,525 - BWI76_RS02085 0.51 -1.2 430,538 - BWI76_RS02085 0.51 -0.2 430,673 - BWI76_RS02085 0.60 +0.4 430,696 + BWI76_RS02085 0.61 +0.6 430,745 - BWI76_RS02085 0.64 +0.4 430,888 + BWI76_RS02085 0.73 -2.0 430,914 + BWI76_RS02085 0.74 -0.0 430,922 - BWI76_RS02085 0.75 +0.6 430,976 - BWI76_RS02085 0.78 -0.5 431,056 - BWI76_RS02085 0.83 -1.8 431,056 - BWI76_RS02085 0.83 -0.3 431,082 - BWI76_RS02085 0.84 -1.0 431,192 + -0.3 431,200 - +1.2 431,265 - +0.0 431,265 - +0.3 431,270 - -1.2 431,323 + -0.6 431,344 + +0.5 431,502 - BWI76_RS02090 0.52 -0.2 431,536 - BWI76_RS02090 0.63 -1.9 431,559 - BWI76_RS02090 0.70 -1.0 431,668 - -0.2 431,726 - +1.6 431,728 + -0.2 431,762 + +2.2 431,770 - -0.1 431,815 + +0.3 431,847 + -0.0 431,901 + -0.4 432,063 + BWI76_RS02095 0.12 -2.0 432,066 - -1.4 432,071 - BWI76_RS02095 0.12 -0.3 432,094 + BWI76_RS02095 0.13 +0.3 432,094 + BWI76_RS02095 0.13 +0.3 432,110 + BWI76_RS02095 0.14 +0.7 432,126 + BWI76_RS02095 0.15 -0.5 432,297 + BWI76_RS02095 0.24 +0.9 432,381 + BWI76_RS02095 0.28 -0.7 432,457 + BWI76_RS02095 0.32 -0.4 432,526 + BWI76_RS02095 0.35 +0.6 432,620 - BWI76_RS02095 0.40 +0.0
Or see this region's nucleotide sequence