Experiment: KLEB24 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26775 and BWI76_RS26780 are separated by 3 nucleotides BWI76_RS26780 and BWI76_RS26785 are separated by 10 nucleotides
BWI76_RS26775: BWI76_RS26775 - endo-1,4-D-glucanase, at 5,549,842 to 5,550,951
_RS26775
BWI76_RS26780: BWI76_RS26780 - cellulose synthase regulator BcsB, at 5,550,955 to 5,553,288
_RS26780
BWI76_RS26785: BWI76_RS26785 - UDP-forming cellulose synthase catalytic subunit, at 5,553,299 to 5,555,917
_RS26785
Position (kb) 5550
5551
5552
5553
5554 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5549.961 kb on - strand, within BWI76_RS26775 at 5550.115 kb on + strand, within BWI76_RS26775 at 5550.227 kb on - strand, within BWI76_RS26775 at 5550.407 kb on - strand, within BWI76_RS26775 at 5550.585 kb on - strand, within BWI76_RS26775 at 5550.594 kb on + strand, within BWI76_RS26775 at 5550.627 kb on - strand, within BWI76_RS26775 at 5550.637 kb on - strand, within BWI76_RS26775 at 5550.639 kb on - strand, within BWI76_RS26775 at 5550.726 kb on + strand, within BWI76_RS26775 at 5550.763 kb on + strand, within BWI76_RS26775 at 5550.768 kb on + strand, within BWI76_RS26775 at 5550.887 kb on + strand at 5551.134 kb on + strand at 5551.540 kb on - strand, within BWI76_RS26780 at 5551.766 kb on + strand, within BWI76_RS26780 at 5551.771 kb on + strand, within BWI76_RS26780 at 5551.771 kb on + strand, within BWI76_RS26780 at 5551.779 kb on - strand, within BWI76_RS26780 at 5551.779 kb on - strand, within BWI76_RS26780 at 5551.790 kb on + strand, within BWI76_RS26780 at 5551.790 kb on + strand, within BWI76_RS26780 at 5551.798 kb on - strand, within BWI76_RS26780 at 5551.940 kb on + strand, within BWI76_RS26780 at 5552.286 kb on - strand, within BWI76_RS26780 at 5552.290 kb on + strand, within BWI76_RS26780 at 5552.295 kb on + strand, within BWI76_RS26780 at 5552.797 kb on - strand, within BWI76_RS26780 at 5552.936 kb on - strand, within BWI76_RS26780 at 5553.030 kb on + strand, within BWI76_RS26780 at 5553.038 kb on - strand, within BWI76_RS26780 at 5553.038 kb on - strand, within BWI76_RS26780 at 5553.048 kb on - strand, within BWI76_RS26780 at 5553.055 kb on - strand at 5553.370 kb on + strand at 5553.442 kb on + strand at 5553.510 kb on + strand at 5553.510 kb on + strand at 5553.549 kb on + strand at 5553.598 kb on + strand, within BWI76_RS26785 at 5553.626 kb on - strand, within BWI76_RS26785 at 5553.648 kb on + strand, within BWI76_RS26785 at 5553.767 kb on - strand, within BWI76_RS26785 at 5553.767 kb on - strand, within BWI76_RS26785 at 5553.846 kb on - strand, within BWI76_RS26785 at 5553.859 kb on + strand, within BWI76_RS26785 at 5553.966 kb on - strand, within BWI76_RS26785 at 5554.076 kb on - strand, within BWI76_RS26785 at 5554.083 kb on + strand, within BWI76_RS26785 at 5554.113 kb on + strand, within BWI76_RS26785 at 5554.124 kb on - strand, within BWI76_RS26785 at 5554.132 kb on - strand, within BWI76_RS26785 at 5554.180 kb on - strand, within BWI76_RS26785 at 5554.190 kb on + strand, within BWI76_RS26785
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB24 MOI 1 remove 5,549,961 - BWI76_RS26775 0.11 -1.4 5,550,115 + BWI76_RS26775 0.25 -0.2 5,550,227 - BWI76_RS26775 0.35 -1.4 5,550,407 - BWI76_RS26775 0.51 -1.3 5,550,585 - BWI76_RS26775 0.67 -0.2 5,550,594 + BWI76_RS26775 0.68 -1.4 5,550,627 - BWI76_RS26775 0.71 -2.2 5,550,637 - BWI76_RS26775 0.72 +0.5 5,550,639 - BWI76_RS26775 0.72 +0.2 5,550,726 + BWI76_RS26775 0.80 -0.4 5,550,763 + BWI76_RS26775 0.83 +2.2 5,550,768 + BWI76_RS26775 0.83 -0.4 5,550,887 + -1.5 5,551,134 + +1.4 5,551,540 - BWI76_RS26780 0.25 +2.0 5,551,766 + BWI76_RS26780 0.35 +2.2 5,551,771 + BWI76_RS26780 0.35 +1.2 5,551,771 + BWI76_RS26780 0.35 +1.2 5,551,779 - BWI76_RS26780 0.35 +1.7 5,551,779 - BWI76_RS26780 0.35 +2.4 5,551,790 + BWI76_RS26780 0.36 +2.2 5,551,790 + BWI76_RS26780 0.36 +1.6 5,551,798 - BWI76_RS26780 0.36 +1.5 5,551,940 + BWI76_RS26780 0.42 +2.0 5,552,286 - BWI76_RS26780 0.57 +1.4 5,552,290 + BWI76_RS26780 0.57 +0.8 5,552,295 + BWI76_RS26780 0.57 +1.0 5,552,797 - BWI76_RS26780 0.79 +0.8 5,552,936 - BWI76_RS26780 0.85 +1.5 5,553,030 + BWI76_RS26780 0.89 +1.9 5,553,038 - BWI76_RS26780 0.89 +1.1 5,553,038 - BWI76_RS26780 0.89 +0.4 5,553,048 - BWI76_RS26780 0.90 +1.8 5,553,055 - +1.5 5,553,370 + +1.0 5,553,442 + +0.0 5,553,510 + +1.4 5,553,510 + +1.0 5,553,549 + +1.2 5,553,598 + BWI76_RS26785 0.11 +1.7 5,553,626 - BWI76_RS26785 0.12 +3.3 5,553,648 + BWI76_RS26785 0.13 +0.7 5,553,767 - BWI76_RS26785 0.18 +2.7 5,553,767 - BWI76_RS26785 0.18 +1.7 5,553,846 - BWI76_RS26785 0.21 +1.3 5,553,859 + BWI76_RS26785 0.21 +1.2 5,553,966 - BWI76_RS26785 0.25 +2.7 5,554,076 - BWI76_RS26785 0.30 +1.8 5,554,083 + BWI76_RS26785 0.30 +2.0 5,554,113 + BWI76_RS26785 0.31 +2.0 5,554,124 - BWI76_RS26785 0.32 +2.1 5,554,132 - BWI76_RS26785 0.32 +1.3 5,554,180 - BWI76_RS26785 0.34 +2.2 5,554,190 + BWI76_RS26785 0.34 +2.2
Or see this region's nucleotide sequence