Experiment: KLEB24 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22295 and BWI76_RS22300 are separated by 212 nucleotides BWI76_RS22300 and BWI76_RS22305 overlap by 8 nucleotides BWI76_RS22305 and BWI76_RS22310 overlap by 4 nucleotides
BWI76_RS22295: BWI76_RS22295 - 6-phospho-beta-glucosidase, at 4,648,310 to 4,649,734
_RS22295
BWI76_RS22300: BWI76_RS22300 - hydrogenase 3 maturation endopeptidase HyCI, at 4,649,947 to 4,650,432
_RS22300
BWI76_RS22305: BWI76_RS22305 - formate hydrogenlyase maturation protein HycH, at 4,650,425 to 4,650,835
_RS22305
BWI76_RS22310: BWI76_RS22310 - hydrogenase 3 small subunit, at 4,650,832 to 4,651,599
_RS22310
Position (kb) 4649
4650
4651 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4649.018 kb on - strand, within BWI76_RS22295 at 4649.040 kb on - strand, within BWI76_RS22295 at 4649.119 kb on - strand, within BWI76_RS22295 at 4649.173 kb on - strand, within BWI76_RS22295 at 4649.251 kb on - strand, within BWI76_RS22295 at 4649.368 kb on + strand, within BWI76_RS22295 at 4649.552 kb on + strand, within BWI76_RS22295 at 4649.559 kb on - strand, within BWI76_RS22295 at 4649.614 kb on + strand at 4649.774 kb on - strand at 4649.995 kb on + strand at 4650.366 kb on - strand, within BWI76_RS22300 at 4650.494 kb on - strand, within BWI76_RS22305 at 4650.990 kb on + strand, within BWI76_RS22310 at 4651.316 kb on + strand, within BWI76_RS22310 at 4651.334 kb on - strand, within BWI76_RS22310 at 4651.414 kb on - strand, within BWI76_RS22310 at 4651.414 kb on - strand, within BWI76_RS22310
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB24 MOI 1 remove 4,649,018 - BWI76_RS22295 0.50 -0.9 4,649,040 - BWI76_RS22295 0.51 -0.9 4,649,119 - BWI76_RS22295 0.57 -0.7 4,649,173 - BWI76_RS22295 0.61 +0.7 4,649,251 - BWI76_RS22295 0.66 -0.7 4,649,368 + BWI76_RS22295 0.74 -1.8 4,649,552 + BWI76_RS22295 0.87 -0.1 4,649,559 - BWI76_RS22295 0.88 -1.3 4,649,614 + +0.4 4,649,774 - -0.2 4,649,995 + -1.1 4,650,366 - BWI76_RS22300 0.86 -0.9 4,650,494 - BWI76_RS22305 0.17 -0.5 4,650,990 + BWI76_RS22310 0.21 +1.8 4,651,316 + BWI76_RS22310 0.63 +0.0 4,651,334 - BWI76_RS22310 0.65 +0.4 4,651,414 - BWI76_RS22310 0.76 +0.4 4,651,414 - BWI76_RS22310 0.76 -0.7
Or see this region's nucleotide sequence