Experiment: KLEB24 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17855 and BWI76_RS17860 are separated by 1 nucleotides BWI76_RS17860 and BWI76_RS17865 are separated by 90 nucleotides
BWI76_RS17855: BWI76_RS17855 - alginate lyase, at 3,712,073 to 3,714,103
_RS17855
BWI76_RS17860: BWI76_RS17860 - YoaH family protein, at 3,714,105 to 3,714,284
_RS17860
BWI76_RS17865: BWI76_RS17865 - aminodeoxychorismate synthase subunit I, at 3,714,375 to 3,715,730
_RS17865
Position (kb)
3714
3715 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3713.394 kb on + strand, within BWI76_RS17855 at 3713.514 kb on + strand, within BWI76_RS17855 at 3713.580 kb on + strand, within BWI76_RS17855 at 3713.800 kb on + strand, within BWI76_RS17855 at 3714.075 kb on + strand at 3714.094 kb on - strand at 3714.194 kb on - strand, within BWI76_RS17860 at 3714.261 kb on - strand at 3714.368 kb on - strand at 3714.368 kb on - strand at 3714.445 kb on + strand at 3714.452 kb on + strand at 3714.618 kb on + strand, within BWI76_RS17865 at 3714.642 kb on - strand, within BWI76_RS17865 at 3714.906 kb on - strand, within BWI76_RS17865 at 3714.927 kb on + strand, within BWI76_RS17865 at 3714.965 kb on + strand, within BWI76_RS17865 at 3714.974 kb on - strand, within BWI76_RS17865 at 3714.975 kb on + strand, within BWI76_RS17865 at 3715.131 kb on - strand, within BWI76_RS17865 at 3715.175 kb on + strand, within BWI76_RS17865 at 3715.221 kb on - strand, within BWI76_RS17865 at 3715.221 kb on - strand, within BWI76_RS17865
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB24 MOI 1 remove 3,713,394 + BWI76_RS17855 0.65 +1.9 3,713,514 + BWI76_RS17855 0.71 +1.9 3,713,580 + BWI76_RS17855 0.74 -0.2 3,713,800 + BWI76_RS17855 0.85 +0.3 3,714,075 + +0.6 3,714,094 - -0.3 3,714,194 - BWI76_RS17860 0.49 -0.9 3,714,261 - -1.0 3,714,368 - -1.7 3,714,368 - +0.1 3,714,445 + +0.5 3,714,452 + -0.6 3,714,618 + BWI76_RS17865 0.18 -0.6 3,714,642 - BWI76_RS17865 0.20 +1.4 3,714,906 - BWI76_RS17865 0.39 +0.2 3,714,927 + BWI76_RS17865 0.41 +1.4 3,714,965 + BWI76_RS17865 0.44 -1.8 3,714,974 - BWI76_RS17865 0.44 +0.1 3,714,975 + BWI76_RS17865 0.44 +0.1 3,715,131 - BWI76_RS17865 0.56 -0.8 3,715,175 + BWI76_RS17865 0.59 +2.6 3,715,221 - BWI76_RS17865 0.62 -0.9 3,715,221 - BWI76_RS17865 0.62 -0.1
Or see this region's nucleotide sequence