Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS13445

Experiment: KLEB24 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS13430 and BWI76_RS13435 are separated by 52 nucleotidesBWI76_RS13435 and BWI76_RS13440 are separated by 196 nucleotidesBWI76_RS13440 and BWI76_RS13445 are separated by 72 nucleotidesBWI76_RS13445 and BWI76_RS13450 are separated by 80 nucleotides BWI76_RS13430: BWI76_RS13430 - hypothetical protein, at 2,802,498 to 2,802,782 _RS13430 BWI76_RS13435: BWI76_RS13435 - hypothetical protein, at 2,802,835 to 2,802,927 _RS13435 BWI76_RS13440: BWI76_RS13440 - DUF2566 domain-containing protein, at 2,803,124 to 2,803,297 _RS13440 BWI76_RS13445: BWI76_RS13445 - alpha/beta hydrolase, at 2,803,370 to 2,804,140 _RS13445 BWI76_RS13450: BWI76_RS13450 - acyl-CoA thioesterase, at 2,804,221 to 2,805,477 _RS13450 Position (kb) 2803 2804 2805Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2802.405 kb on + strandat 2802.406 kb on - strandat 2802.406 kb on - strandat 2802.455 kb on - strandat 2802.470 kb on - strandat 2802.482 kb on - strandat 2802.658 kb on + strand, within BWI76_RS13430at 2802.898 kb on - strand, within BWI76_RS13435at 2802.957 kb on + strandat 2802.968 kb on + strandat 2803.061 kb on + strandat 2803.388 kb on + strandat 2803.593 kb on - strand, within BWI76_RS13445at 2803.782 kb on - strand, within BWI76_RS13445at 2804.244 kb on - strandat 2804.264 kb on - strandat 2804.306 kb on + strandat 2804.578 kb on + strand, within BWI76_RS13450at 2804.645 kb on + strand, within BWI76_RS13450at 2804.797 kb on + strand, within BWI76_RS13450at 2804.846 kb on - strand, within BWI76_RS13450at 2804.846 kb on - strand, within BWI76_RS13450

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB24 MOI 1
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2,802,405 + +0.1
2,802,406 - +1.3
2,802,406 - +1.6
2,802,455 - -0.2
2,802,470 - -0.8
2,802,482 - -0.2
2,802,658 + BWI76_RS13430 0.56 -0.7
2,802,898 - BWI76_RS13435 0.68 +0.8
2,802,957 + +0.1
2,802,968 + -2.0
2,803,061 + +2.4
2,803,388 + -0.3
2,803,593 - BWI76_RS13445 0.29 +1.3
2,803,782 - BWI76_RS13445 0.53 -1.4
2,804,244 - -1.2
2,804,264 - -1.0
2,804,306 + +0.8
2,804,578 + BWI76_RS13450 0.28 -1.5
2,804,645 + BWI76_RS13450 0.34 -0.0
2,804,797 + BWI76_RS13450 0.46 -3.1
2,804,846 - BWI76_RS13450 0.50 -0.2
2,804,846 - BWI76_RS13450 0.50 +0.2

Or see this region's nucleotide sequence