Experiment: KLEB24 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13430 and BWI76_RS13435 are separated by 52 nucleotides BWI76_RS13435 and BWI76_RS13440 are separated by 196 nucleotides BWI76_RS13440 and BWI76_RS13445 are separated by 72 nucleotides BWI76_RS13445 and BWI76_RS13450 are separated by 80 nucleotides
BWI76_RS13430: BWI76_RS13430 - hypothetical protein, at 2,802,498 to 2,802,782
_RS13430
BWI76_RS13435: BWI76_RS13435 - hypothetical protein, at 2,802,835 to 2,802,927
_RS13435
BWI76_RS13440: BWI76_RS13440 - DUF2566 domain-containing protein, at 2,803,124 to 2,803,297
_RS13440
BWI76_RS13445: BWI76_RS13445 - alpha/beta hydrolase, at 2,803,370 to 2,804,140
_RS13445
BWI76_RS13450: BWI76_RS13450 - acyl-CoA thioesterase, at 2,804,221 to 2,805,477
_RS13450
Position (kb)
2803
2804
2805 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2802.405 kb on + strand at 2802.406 kb on - strand at 2802.406 kb on - strand at 2802.455 kb on - strand at 2802.470 kb on - strand at 2802.482 kb on - strand at 2802.658 kb on + strand, within BWI76_RS13430 at 2802.898 kb on - strand, within BWI76_RS13435 at 2802.957 kb on + strand at 2802.968 kb on + strand at 2803.061 kb on + strand at 2803.388 kb on + strand at 2803.593 kb on - strand, within BWI76_RS13445 at 2803.782 kb on - strand, within BWI76_RS13445 at 2804.244 kb on - strand at 2804.264 kb on - strand at 2804.306 kb on + strand at 2804.578 kb on + strand, within BWI76_RS13450 at 2804.645 kb on + strand, within BWI76_RS13450 at 2804.797 kb on + strand, within BWI76_RS13450 at 2804.846 kb on - strand, within BWI76_RS13450 at 2804.846 kb on - strand, within BWI76_RS13450
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB24 MOI 1 remove 2,802,405 + +0.1 2,802,406 - +1.3 2,802,406 - +1.6 2,802,455 - -0.2 2,802,470 - -0.8 2,802,482 - -0.2 2,802,658 + BWI76_RS13430 0.56 -0.7 2,802,898 - BWI76_RS13435 0.68 +0.8 2,802,957 + +0.1 2,802,968 + -2.0 2,803,061 + +2.4 2,803,388 + -0.3 2,803,593 - BWI76_RS13445 0.29 +1.3 2,803,782 - BWI76_RS13445 0.53 -1.4 2,804,244 - -1.2 2,804,264 - -1.0 2,804,306 + +0.8 2,804,578 + BWI76_RS13450 0.28 -1.5 2,804,645 + BWI76_RS13450 0.34 -0.0 2,804,797 + BWI76_RS13450 0.46 -3.1 2,804,846 - BWI76_RS13450 0.50 -0.2 2,804,846 - BWI76_RS13450 0.50 +0.2
Or see this region's nucleotide sequence