Experiment: KLEB24 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11275 and BWI76_RS11280 overlap by 4 nucleotides BWI76_RS11280 and BWI76_RS11285 are separated by 84 nucleotides BWI76_RS11285 and BWI76_RS11290 are separated by 100 nucleotides
BWI76_RS11275: BWI76_RS11275 - sensor protein PhoQ, at 2,377,821 to 2,379,287
_RS11275
BWI76_RS11280: BWI76_RS11280 - two-component system response regulator PhoP, at 2,379,284 to 2,379,958
_RS11280
BWI76_RS11285: BWI76_RS11285 - transcriptional regulator, at 2,380,043 to 2,380,489
_RS11285
BWI76_RS11290: BWI76_RS11290 - MFS transporter, at 2,380,590 to 2,381,819
_RS11290
Position (kb)
2380
2381 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2379.106 kb on + strand, within BWI76_RS11275 at 2379.930 kb on + strand at 2380.386 kb on - strand, within BWI76_RS11285 at 2380.818 kb on + strand, within BWI76_RS11290 at 2380.884 kb on - strand, within BWI76_RS11290 at 2381.047 kb on - strand, within BWI76_RS11290 at 2381.123 kb on - strand, within BWI76_RS11290 at 2381.207 kb on - strand, within BWI76_RS11290 at 2381.207 kb on - strand, within BWI76_RS11290
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB24 MOI 1 remove 2,379,106 + BWI76_RS11275 0.88 -1.8 2,379,930 + -0.4 2,380,386 - BWI76_RS11285 0.77 +2.5 2,380,818 + BWI76_RS11290 0.19 -1.3 2,380,884 - BWI76_RS11290 0.24 +0.3 2,381,047 - BWI76_RS11290 0.37 -0.3 2,381,123 - BWI76_RS11290 0.43 +0.5 2,381,207 - BWI76_RS11290 0.50 +0.0 2,381,207 - BWI76_RS11290 0.50 -0.3
Or see this region's nucleotide sequence