Experiment: KLEB24 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10670 and BWI76_RS10675 are separated by 308 nucleotides BWI76_RS10675 and BWI76_RS10680 are separated by 32 nucleotides
BWI76_RS10670: BWI76_RS10670 - PTS fructose transporter subunit IIC, at 2,252,753 to 2,253,829
_RS10670
BWI76_RS10675: BWI76_RS10675 - phosphoenolpyruvate--protein phosphotransferase, at 2,254,138 to 2,256,639
_RS10675
BWI76_RS10680: BWI76_RS10680 - tartrate dehydrogenase, at 2,256,672 to 2,257,790
_RS10680
Position (kb)
2254
2255
2256
2257 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2253.276 kb on + strand, within BWI76_RS10670 at 2253.676 kb on + strand, within BWI76_RS10670 at 2253.783 kb on - strand at 2254.178 kb on + strand at 2254.365 kb on - strand at 2254.570 kb on - strand, within BWI76_RS10675 at 2254.815 kb on - strand, within BWI76_RS10675 at 2255.217 kb on + strand, within BWI76_RS10675 at 2255.566 kb on + strand, within BWI76_RS10675 at 2255.574 kb on - strand, within BWI76_RS10675 at 2255.908 kb on - strand, within BWI76_RS10675 at 2256.226 kb on - strand, within BWI76_RS10675 at 2256.245 kb on + strand, within BWI76_RS10675 at 2256.245 kb on + strand, within BWI76_RS10675 at 2256.253 kb on - strand, within BWI76_RS10675 at 2256.299 kb on + strand, within BWI76_RS10675 at 2256.634 kb on - strand at 2257.058 kb on + strand, within BWI76_RS10680 at 2257.097 kb on + strand, within BWI76_RS10680
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB24 MOI 1 remove 2,253,276 + BWI76_RS10670 0.49 -1.0 2,253,676 + BWI76_RS10670 0.86 -0.1 2,253,783 - -0.2 2,254,178 + -0.7 2,254,365 - -1.0 2,254,570 - BWI76_RS10675 0.17 -1.3 2,254,815 - BWI76_RS10675 0.27 +0.0 2,255,217 + BWI76_RS10675 0.43 -0.6 2,255,566 + BWI76_RS10675 0.57 -1.3 2,255,574 - BWI76_RS10675 0.57 -0.5 2,255,908 - BWI76_RS10675 0.71 -0.6 2,256,226 - BWI76_RS10675 0.83 -3.0 2,256,245 + BWI76_RS10675 0.84 -0.8 2,256,245 + BWI76_RS10675 0.84 -1.1 2,256,253 - BWI76_RS10675 0.85 +1.8 2,256,299 + BWI76_RS10675 0.86 -0.5 2,256,634 - +1.0 2,257,058 + BWI76_RS10680 0.34 -1.0 2,257,097 + BWI76_RS10680 0.38 -0.0
Or see this region's nucleotide sequence