Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10585

Experiment: KLEB24 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10575 and BWI76_RS10580 overlap by 1 nucleotidesBWI76_RS10580 and BWI76_RS10585 overlap by 20 nucleotidesBWI76_RS10585 and BWI76_RS10590 are separated by 10 nucleotides BWI76_RS10575: BWI76_RS10575 - polar amino acid ABC transporter permease, at 2,233,665 to 2,234,330 _RS10575 BWI76_RS10580: BWI76_RS10580 - amino acid ABC transporter permease, at 2,234,330 to 2,234,986 _RS10580 BWI76_RS10585: BWI76_RS10585 - amino acid ABC transporter ATP-binding protein, at 2,234,967 to 2,235,707 _RS10585 BWI76_RS10590: BWI76_RS10590 - class V aminotransferase, at 2,235,718 to 2,236,959 _RS10590 Position (kb) 2234 2235 2236Strain fitness (log2 ratio) -2 -1 0 1 2at 2233.978 kb on + strand, within BWI76_RS10575at 2234.275 kb on - strandat 2234.506 kb on + strand, within BWI76_RS10580at 2234.514 kb on - strand, within BWI76_RS10580at 2234.855 kb on - strand, within BWI76_RS10580at 2235.150 kb on - strandat 2235.161 kb on + strand, within BWI76_RS10585at 2235.391 kb on + strand, within BWI76_RS10585at 2235.650 kb on + strandat 2235.709 kb on + strandat 2235.765 kb on + strandat 2236.016 kb on + strand, within BWI76_RS10590at 2236.465 kb on + strand, within BWI76_RS10590at 2236.477 kb on + strand, within BWI76_RS10590at 2236.631 kb on - strand, within BWI76_RS10590

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB24 MOI 1
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2,233,978 + BWI76_RS10575 0.47 -1.2
2,234,275 - -1.9
2,234,506 + BWI76_RS10580 0.27 -0.6
2,234,514 - BWI76_RS10580 0.28 -0.5
2,234,855 - BWI76_RS10580 0.80 -0.2
2,235,150 - -0.6
2,235,161 + BWI76_RS10585 0.26 -0.8
2,235,391 + BWI76_RS10585 0.57 -1.1
2,235,650 + -0.5
2,235,709 + -0.3
2,235,765 + +2.1
2,236,016 + BWI76_RS10590 0.24 -0.7
2,236,465 + BWI76_RS10590 0.60 +0.0
2,236,477 + BWI76_RS10590 0.61 -0.6
2,236,631 - BWI76_RS10590 0.74 +0.2

Or see this region's nucleotide sequence