Experiment: KLEB24 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10480 and BWI76_RS10485 are separated by 10 nucleotides BWI76_RS10485 and BWI76_RS10490 are separated by 13 nucleotides BWI76_RS10490 and BWI76_RS10495 are separated by 125 nucleotides
BWI76_RS10480: BWI76_RS10480 - amino acid ABC transporter substrate-binding protein, at 2,214,370 to 2,215,398
_RS10480
BWI76_RS10485: BWI76_RS10485 - (hypo)xanthine hydroxylase reductase, at 2,215,409 to 2,216,377
_RS10485
BWI76_RS10490: BWI76_RS10490 - vanillate O-demethylase oxygenase subunit, at 2,216,391 to 2,217,428
_RS10490
BWI76_RS10495: BWI76_RS10495 - LysR family transcriptional regulator, at 2,217,554 to 2,218,492
_RS10495
Position (kb)
2216
2217
2218 Strain fitness (log2 ratio)
-1
0
1
2 at 2215.464 kb on - strand at 2215.566 kb on + strand, within BWI76_RS10485 at 2215.611 kb on - strand, within BWI76_RS10485 at 2216.066 kb on + strand, within BWI76_RS10485 at 2216.201 kb on + strand, within BWI76_RS10485 at 2216.552 kb on + strand, within BWI76_RS10490 at 2216.552 kb on + strand, within BWI76_RS10490 at 2216.560 kb on - strand, within BWI76_RS10490 at 2216.841 kb on - strand, within BWI76_RS10490 at 2216.881 kb on + strand, within BWI76_RS10490 at 2217.254 kb on + strand, within BWI76_RS10490 at 2217.431 kb on + strand at 2217.431 kb on - strand at 2217.915 kb on + strand, within BWI76_RS10495 at 2218.027 kb on - strand, within BWI76_RS10495 at 2218.128 kb on + strand, within BWI76_RS10495 at 2218.303 kb on + strand, within BWI76_RS10495 at 2218.308 kb on + strand, within BWI76_RS10495 at 2218.362 kb on + strand, within BWI76_RS10495 at 2218.367 kb on + strand, within BWI76_RS10495
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB24 MOI 1 remove 2,215,464 - +0.5 2,215,566 + BWI76_RS10485 0.16 -0.4 2,215,611 - BWI76_RS10485 0.21 -1.1 2,216,066 + BWI76_RS10485 0.68 -0.4 2,216,201 + BWI76_RS10485 0.82 -1.7 2,216,552 + BWI76_RS10490 0.16 -1.7 2,216,552 + BWI76_RS10490 0.16 -0.1 2,216,560 - BWI76_RS10490 0.16 -0.8 2,216,841 - BWI76_RS10490 0.43 -0.6 2,216,881 + BWI76_RS10490 0.47 -0.6 2,217,254 + BWI76_RS10490 0.83 -0.6 2,217,431 + -1.5 2,217,431 - +0.1 2,217,915 + BWI76_RS10495 0.38 -0.4 2,218,027 - BWI76_RS10495 0.50 +2.4 2,218,128 + BWI76_RS10495 0.61 -0.3 2,218,303 + BWI76_RS10495 0.80 +0.8 2,218,308 + BWI76_RS10495 0.80 -0.4 2,218,362 + BWI76_RS10495 0.86 -0.3 2,218,367 + BWI76_RS10495 0.87 -0.5
Or see this region's nucleotide sequence