Experiment: KLEB24 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10395 and BWI76_RS10400 are separated by 355 nucleotides BWI76_RS10400 and BWI76_RS10405 are separated by 125 nucleotides BWI76_RS10405 and BWI76_RS10410 are separated by 234 nucleotides BWI76_RS10410 and BWI76_RS10415 are separated by 91 nucleotides
BWI76_RS10395: BWI76_RS10395 - cold-shock protein, at 2,201,107 to 2,201,319
_RS10395
BWI76_RS10400: BWI76_RS10400 - hypothetical protein, at 2,201,675 to 2,201,860
_RS10400
BWI76_RS10405: BWI76_RS10405 - cold-shock protein, at 2,201,986 to 2,202,207
_RS10405
BWI76_RS10410: BWI76_RS10410 - hypothetical protein, at 2,202,442 to 2,202,702
_RS10410
BWI76_RS10415: BWI76_RS10415 - endoribonuclease L-PSP, at 2,202,794 to 2,203,171
_RS10415
Position (kb)
2201
2202 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2200.694 kb on - strand at 2200.694 kb on - strand at 2200.784 kb on - strand at 2200.866 kb on + strand at 2200.875 kb on + strand at 2200.975 kb on + strand at 2200.975 kb on + strand at 2200.975 kb on + strand at 2200.975 kb on + strand at 2200.975 kb on + strand at 2201.056 kb on - strand at 2201.056 kb on - strand at 2201.056 kb on - strand at 2201.067 kb on - strand at 2201.085 kb on - strand at 2201.114 kb on + strand at 2201.114 kb on + strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.127 kb on - strand at 2201.178 kb on - strand, within BWI76_RS10395 at 2201.183 kb on - strand, within BWI76_RS10395 at 2201.190 kb on - strand, within BWI76_RS10395 at 2201.248 kb on + strand at 2201.255 kb on - strand, within BWI76_RS10395 at 2201.256 kb on - strand, within BWI76_RS10395 at 2201.256 kb on - strand, within BWI76_RS10395 at 2201.270 kb on - strand at 2201.270 kb on - strand, within BWI76_RS10395 at 2201.270 kb on - strand, within BWI76_RS10395 at 2201.288 kb on - strand, within BWI76_RS10395 at 2201.344 kb on - strand at 2201.344 kb on - strand at 2201.344 kb on - strand at 2201.345 kb on - strand at 2201.345 kb on - strand at 2201.379 kb on - strand at 2201.425 kb on + strand at 2201.425 kb on + strand at 2201.427 kb on + strand at 2201.433 kb on - strand at 2201.471 kb on + strand at 2201.471 kb on + strand at 2201.550 kb on + strand at 2201.573 kb on + strand at 2201.584 kb on + strand at 2201.591 kb on + strand at 2201.639 kb on + strand at 2201.698 kb on + strand, within BWI76_RS10400 at 2201.698 kb on + strand, within BWI76_RS10400 at 2201.706 kb on - strand, within BWI76_RS10400 at 2201.711 kb on - strand, within BWI76_RS10400 at 2201.724 kb on - strand, within BWI76_RS10400 at 2201.765 kb on + strand, within BWI76_RS10400 at 2201.877 kb on + strand at 2201.933 kb on + strand at 2201.936 kb on - strand at 2201.941 kb on - strand at 2201.941 kb on - strand at 2201.941 kb on - strand at 2201.941 kb on - strand at 2201.965 kb on - strand at 2201.972 kb on + strand at 2201.986 kb on - strand at 2202.004 kb on + strand at 2202.007 kb on + strand at 2202.012 kb on - strand, within BWI76_RS10405 at 2202.017 kb on + strand, within BWI76_RS10405 at 2202.025 kb on - strand, within BWI76_RS10405 at 2202.027 kb on + strand, within BWI76_RS10405 at 2202.071 kb on - strand, within BWI76_RS10405 at 2202.082 kb on - strand, within BWI76_RS10405 at 2202.084 kb on + strand, within BWI76_RS10405 at 2202.086 kb on + strand, within BWI76_RS10405 at 2202.150 kb on + strand, within BWI76_RS10405 at 2202.153 kb on + strand, within BWI76_RS10405 at 2202.160 kb on + strand, within BWI76_RS10405 at 2202.161 kb on - strand, within BWI76_RS10405 at 2202.161 kb on - strand, within BWI76_RS10405 at 2202.170 kb on + strand, within BWI76_RS10405 at 2202.171 kb on - strand, within BWI76_RS10405 at 2202.175 kb on + strand, within BWI76_RS10405 at 2202.183 kb on - strand, within BWI76_RS10405 at 2202.183 kb on - strand, within BWI76_RS10405 at 2202.185 kb on + strand at 2202.185 kb on + strand at 2202.185 kb on + strand at 2202.185 kb on + strand at 2202.185 kb on + strand at 2202.185 kb on + strand at 2202.185 kb on + strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.233 kb on + strand at 2202.233 kb on + strand at 2202.233 kb on + strand at 2202.233 kb on + strand at 2202.233 kb on + strand at 2202.241 kb on - strand at 2202.241 kb on - strand at 2202.254 kb on + strand at 2202.268 kb on + strand at 2202.273 kb on + strand at 2202.284 kb on + strand at 2202.299 kb on - strand at 2202.309 kb on + strand at 2202.309 kb on + strand at 2202.389 kb on + strand at 2202.394 kb on + strand at 2202.394 kb on + strand at 2202.442 kb on - strand at 2202.498 kb on - strand, within BWI76_RS10410 at 2202.610 kb on - strand, within BWI76_RS10410 at 2202.806 kb on - strand at 2202.835 kb on + strand, within BWI76_RS10415 at 2202.845 kb on + strand, within BWI76_RS10415
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB24 MOI 1 remove 2,200,694 - +0.4 2,200,694 - +0.3 2,200,784 - -1.9 2,200,866 + +0.4 2,200,875 + +1.0 2,200,975 + +0.6 2,200,975 + -0.6 2,200,975 + -0.2 2,200,975 + +0.5 2,200,975 + +0.4 2,201,056 - -0.3 2,201,056 - +0.5 2,201,056 - +0.2 2,201,067 - -0.2 2,201,085 - -0.7 2,201,114 + +0.4 2,201,114 + +1.4 2,201,122 - -0.6 2,201,122 - +0.8 2,201,122 - -0.1 2,201,122 - -0.8 2,201,122 - -0.7 2,201,122 - -0.0 2,201,122 - -0.0 2,201,122 - +0.5 2,201,122 - -1.4 2,201,122 - +1.2 2,201,122 - +1.1 2,201,122 - +0.3 2,201,122 - -0.9 2,201,122 - -0.1 2,201,127 - +0.1 2,201,178 - BWI76_RS10395 0.33 +0.7 2,201,183 - BWI76_RS10395 0.36 -1.0 2,201,190 - BWI76_RS10395 0.39 -1.3 2,201,248 + +4.0 2,201,255 - BWI76_RS10395 0.69 -0.9 2,201,256 - BWI76_RS10395 0.70 -1.0 2,201,256 - BWI76_RS10395 0.70 +0.8 2,201,270 - -1.9 2,201,270 - BWI76_RS10395 0.77 +0.4 2,201,270 - BWI76_RS10395 0.77 +0.1 2,201,288 - BWI76_RS10395 0.85 +2.2 2,201,344 - -0.7 2,201,344 - -1.6 2,201,344 - +0.7 2,201,345 - -0.5 2,201,345 - +1.3 2,201,379 - +0.6 2,201,425 + -1.6 2,201,425 + +2.6 2,201,427 + +0.1 2,201,433 - +0.7 2,201,471 + -0.6 2,201,471 + +0.4 2,201,550 + +0.4 2,201,573 + +0.5 2,201,584 + +0.4 2,201,591 + +0.8 2,201,639 + +0.6 2,201,698 + BWI76_RS10400 0.12 +2.2 2,201,698 + BWI76_RS10400 0.12 -0.6 2,201,706 - BWI76_RS10400 0.17 -0.6 2,201,711 - BWI76_RS10400 0.19 -1.5 2,201,724 - BWI76_RS10400 0.26 +0.4 2,201,765 + BWI76_RS10400 0.48 -1.8 2,201,877 + -1.0 2,201,933 + +0.9 2,201,936 - -1.9 2,201,941 - -0.2 2,201,941 - -0.1 2,201,941 - +0.7 2,201,941 - +0.6 2,201,965 - +0.7 2,201,972 + -3.7 2,201,986 - -0.8 2,202,004 + -1.9 2,202,007 + -2.4 2,202,012 - BWI76_RS10405 0.12 +0.4 2,202,017 + BWI76_RS10405 0.14 -1.6 2,202,025 - BWI76_RS10405 0.18 -0.6 2,202,027 + BWI76_RS10405 0.18 -2.2 2,202,071 - BWI76_RS10405 0.38 -1.0 2,202,082 - BWI76_RS10405 0.43 -2.0 2,202,084 + BWI76_RS10405 0.44 +0.6 2,202,086 + BWI76_RS10405 0.45 +1.0 2,202,150 + BWI76_RS10405 0.74 +1.2 2,202,153 + BWI76_RS10405 0.75 -1.0 2,202,160 + BWI76_RS10405 0.78 +1.4 2,202,161 - BWI76_RS10405 0.79 -1.2 2,202,161 - BWI76_RS10405 0.79 -0.3 2,202,170 + BWI76_RS10405 0.83 -0.6 2,202,171 - BWI76_RS10405 0.83 +0.1 2,202,175 + BWI76_RS10405 0.85 -0.6 2,202,183 - BWI76_RS10405 0.89 +0.5 2,202,183 - BWI76_RS10405 0.89 +0.3 2,202,185 + +1.4 2,202,185 + -0.5 2,202,185 + -0.4 2,202,185 + -1.3 2,202,185 + +1.7 2,202,185 + -2.4 2,202,185 + -0.0 2,202,193 - -0.4 2,202,193 - -0.2 2,202,193 - -0.9 2,202,193 - -0.2 2,202,193 - -0.6 2,202,193 - -0.1 2,202,193 - +0.4 2,202,193 - +0.9 2,202,193 - -0.7 2,202,193 - -0.2 2,202,193 - +0.2 2,202,193 - -0.2 2,202,193 - +0.0 2,202,233 + -0.8 2,202,233 + -0.4 2,202,233 + +1.2 2,202,233 + +2.1 2,202,233 + -0.8 2,202,241 - +1.6 2,202,241 - -1.2 2,202,254 + +0.6 2,202,268 + +0.8 2,202,273 + -2.4 2,202,284 + +0.1 2,202,299 - -1.9 2,202,309 + +0.8 2,202,309 + +0.5 2,202,389 + +0.8 2,202,394 + -0.5 2,202,394 + +0.4 2,202,442 - -0.0 2,202,498 - BWI76_RS10410 0.21 +0.6 2,202,610 - BWI76_RS10410 0.64 -1.5 2,202,806 - -1.6 2,202,835 + BWI76_RS10415 0.11 -0.8 2,202,845 + BWI76_RS10415 0.13 -0.0
Or see this region's nucleotide sequence