Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS09815

Experiment: KLEB24 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS09805 and BWI76_RS09810 are separated by 52 nucleotidesBWI76_RS09810 and BWI76_RS09815 overlap by 4 nucleotidesBWI76_RS09815 and BWI76_RS09820 are separated by 141 nucleotides BWI76_RS09805: BWI76_RS09805 - hypothetical protein, at 2,057,907 to 2,059,136 _RS09805 BWI76_RS09810: BWI76_RS09810 - hypothetical protein, at 2,059,189 to 2,059,371 _RS09810 BWI76_RS09815: BWI76_RS09815 - 3-deoxy-manno-octulosonate cytidylyltransferase, at 2,059,368 to 2,060,114 _RS09815 BWI76_RS09820: BWI76_RS09820 - hypothetical protein, at 2,060,256 to 2,061,149 _RS09820 Position (kb) 2059 2060 2061Strain fitness (log2 ratio) -1 0 1at 2058.853 kb on - strand, within BWI76_RS09805at 2058.993 kb on - strand, within BWI76_RS09805at 2060.371 kb on + strand, within BWI76_RS09820at 2060.689 kb on - strand, within BWI76_RS09820at 2060.851 kb on + strand, within BWI76_RS09820at 2060.870 kb on + strand, within BWI76_RS09820at 2060.915 kb on + strand, within BWI76_RS09820

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB24 MOI 1
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2,058,853 - BWI76_RS09805 0.77 +0.1
2,058,993 - BWI76_RS09805 0.88 +1.0
2,060,371 + BWI76_RS09820 0.13 +1.5
2,060,689 - BWI76_RS09820 0.48 +0.4
2,060,851 + BWI76_RS09820 0.67 +1.2
2,060,870 + BWI76_RS09820 0.69 +0.1
2,060,915 + BWI76_RS09820 0.74 +1.0

Or see this region's nucleotide sequence