Experiment: KLEB24 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07845 and BWI76_RS07850 are separated by 145 nucleotides BWI76_RS07850 and BWI76_RS07855 overlap by 4 nucleotides
BWI76_RS07845: BWI76_RS07845 - LacI family transcriptional regulator, at 1,664,015 to 1,665,004
_RS07845
BWI76_RS07850: BWI76_RS07850 - putative cytosine deaminase-related metal-dependent hydrolase, at 1,665,150 to 1,666,388
_RS07850
BWI76_RS07855: BWI76_RS07855 - PucR family transcriptional regulator, at 1,666,385 to 1,667,620
_RS07855
Position (kb)
1665
1666
1667 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1664.429 kb on - strand, within BWI76_RS07845 at 1664.872 kb on - strand, within BWI76_RS07845 at 1664.928 kb on - strand at 1665.026 kb on - strand at 1665.090 kb on + strand at 1665.183 kb on - strand at 1665.221 kb on - strand at 1665.250 kb on - strand at 1665.323 kb on + strand, within BWI76_RS07850 at 1665.432 kb on - strand, within BWI76_RS07850 at 1665.631 kb on - strand, within BWI76_RS07850 at 1665.681 kb on - strand, within BWI76_RS07850 at 1665.762 kb on + strand, within BWI76_RS07850 at 1665.921 kb on + strand, within BWI76_RS07850 at 1666.045 kb on + strand, within BWI76_RS07850 at 1666.548 kb on + strand, within BWI76_RS07855 at 1666.669 kb on + strand at 1667.291 kb on - strand, within BWI76_RS07855
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB24 MOI 1 remove 1,664,429 - BWI76_RS07845 0.42 +1.1 1,664,872 - BWI76_RS07845 0.87 -0.2 1,664,928 - +0.0 1,665,026 - -1.0 1,665,090 + -0.9 1,665,183 - -0.1 1,665,221 - -1.0 1,665,250 - +2.2 1,665,323 + BWI76_RS07850 0.14 +0.8 1,665,432 - BWI76_RS07850 0.23 -1.1 1,665,631 - BWI76_RS07850 0.39 -0.5 1,665,681 - BWI76_RS07850 0.43 -0.9 1,665,762 + BWI76_RS07850 0.49 -0.7 1,665,921 + BWI76_RS07850 0.62 -0.7 1,666,045 + BWI76_RS07850 0.72 -1.8 1,666,548 + BWI76_RS07855 0.13 -0.3 1,666,669 + -0.4 1,667,291 - BWI76_RS07855 0.73 -1.5
Or see this region's nucleotide sequence