Experiment: KLEB24 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06555 and BWI76_RS06560 are separated by 201 nucleotides BWI76_RS06560 and BWI76_RS06565 are separated by 66 nucleotides BWI76_RS06565 and BWI76_RS06570 are separated by 126 nucleotides BWI76_RS06570 and BWI76_RS06575 are separated by 47 nucleotides
BWI76_RS06555: BWI76_RS06555 - thioesterase superfamily protein, at 1,378,249 to 1,378,650
_RS06555
BWI76_RS06560: BWI76_RS06560 - 7-cyano-7-deazaguanine synthase QueC, at 1,378,852 to 1,379,547
_RS06560
BWI76_RS06565: BWI76_RS06565 - putative periplasmic solute-binding protein, at 1,379,614 to 1,381,314
_RS06565
BWI76_RS06570: BWI76_RS06570 - thiamin pyrimidine pyrophosphate hydrolase, at 1,381,441 to 1,382,259
_RS06570
BWI76_RS06575: BWI76_RS06575 - cysteine synthase, at 1,382,307 to 1,383,359
_RS06575
Position (kb) 1379
1380
1381
1382 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1378.745 kb on + strand at 1378.987 kb on + strand, within BWI76_RS06560 at 1378.992 kb on + strand, within BWI76_RS06560 at 1379.119 kb on + strand, within BWI76_RS06560 at 1379.156 kb on - strand, within BWI76_RS06560 at 1379.766 kb on - strand at 1379.929 kb on - strand, within BWI76_RS06565 at 1380.101 kb on - strand, within BWI76_RS06565 at 1380.215 kb on - strand, within BWI76_RS06565 at 1380.284 kb on - strand, within BWI76_RS06565 at 1380.416 kb on - strand, within BWI76_RS06565 at 1380.560 kb on - strand, within BWI76_RS06565 at 1380.610 kb on + strand, within BWI76_RS06565 at 1380.742 kb on + strand, within BWI76_RS06565 at 1380.755 kb on - strand, within BWI76_RS06565 at 1380.799 kb on + strand, within BWI76_RS06565 at 1380.939 kb on - strand, within BWI76_RS06565 at 1381.139 kb on + strand, within BWI76_RS06565 at 1381.195 kb on + strand at 1381.317 kb on - strand at 1381.327 kb on - strand at 1381.672 kb on - strand, within BWI76_RS06570 at 1382.023 kb on + strand, within BWI76_RS06570
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB24 MOI 1 remove 1,378,745 + -2.3 1,378,987 + BWI76_RS06560 0.19 +1.3 1,378,992 + BWI76_RS06560 0.20 +3.3 1,379,119 + BWI76_RS06560 0.38 -1.9 1,379,156 - BWI76_RS06560 0.44 +2.5 1,379,766 - +0.6 1,379,929 - BWI76_RS06565 0.19 +0.0 1,380,101 - BWI76_RS06565 0.29 -0.5 1,380,215 - BWI76_RS06565 0.35 +0.2 1,380,284 - BWI76_RS06565 0.39 -0.3 1,380,416 - BWI76_RS06565 0.47 -0.8 1,380,560 - BWI76_RS06565 0.56 +0.9 1,380,610 + BWI76_RS06565 0.59 +0.7 1,380,742 + BWI76_RS06565 0.66 +1.0 1,380,755 - BWI76_RS06565 0.67 +0.7 1,380,799 + BWI76_RS06565 0.70 +0.3 1,380,939 - BWI76_RS06565 0.78 +0.7 1,381,139 + BWI76_RS06565 0.90 +0.5 1,381,195 + +0.6 1,381,317 - -0.1 1,381,327 - +0.9 1,381,672 - BWI76_RS06570 0.28 +0.3 1,382,023 + BWI76_RS06570 0.71 +0.7
Or see this region's nucleotide sequence