Experiment: KLEB24 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06445 and BWI76_RS06450 are separated by 10 nucleotides BWI76_RS06450 and BWI76_RS06455 are separated by 13 nucleotides BWI76_RS06455 and BWI76_RS06460 overlap by 4 nucleotides BWI76_RS06460 and BWI76_RS06465 are separated by 153 nucleotides
BWI76_RS06445: BWI76_RS06445 - FAD-dependent oxidoreductase, at 1,354,409 to 1,356,112
_RS06445
BWI76_RS06450: BWI76_RS06450 - NIPSNAP family protein, at 1,356,123 to 1,356,443
_RS06450
BWI76_RS06455: BWI76_RS06455 - 3-oxosteroid 1-dehydrogenase, at 1,356,457 to 1,358,133
_RS06455
BWI76_RS06460: BWI76_RS06460 - shikimate dehydrogenase, at 1,358,130 to 1,358,951
_RS06460
BWI76_RS06465: BWI76_RS06465 - IclR family transcriptional regulator, at 1,359,105 to 1,359,863
_RS06465
Position (kb) 1356
1357
1358
1359 Strain fitness (log2 ratio) -1
0
1
2
3 at 1355.841 kb on - strand, within BWI76_RS06445 at 1356.277 kb on + strand, within BWI76_RS06450 at 1356.282 kb on + strand, within BWI76_RS06450 at 1356.418 kb on + strand at 1356.506 kb on - strand at 1356.552 kb on - strand at 1356.552 kb on - strand at 1356.591 kb on - strand at 1356.647 kb on - strand, within BWI76_RS06455 at 1356.678 kb on + strand, within BWI76_RS06455 at 1356.806 kb on + strand, within BWI76_RS06455 at 1357.312 kb on + strand, within BWI76_RS06455 at 1357.312 kb on + strand, within BWI76_RS06455 at 1357.368 kb on - strand, within BWI76_RS06455 at 1357.593 kb on + strand, within BWI76_RS06455 at 1357.601 kb on - strand, within BWI76_RS06455 at 1357.803 kb on + strand, within BWI76_RS06455 at 1357.868 kb on + strand, within BWI76_RS06455 at 1358.004 kb on + strand at 1358.076 kb on - strand at 1358.107 kb on - strand at 1358.214 kb on + strand, within BWI76_RS06460 at 1358.222 kb on - strand, within BWI76_RS06460 at 1358.255 kb on - strand, within BWI76_RS06460 at 1358.275 kb on + strand, within BWI76_RS06460 at 1358.498 kb on + strand, within BWI76_RS06460 at 1358.530 kb on - strand, within BWI76_RS06460 at 1358.747 kb on + strand, within BWI76_RS06460
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB24 MOI 1 remove 1,355,841 - BWI76_RS06445 0.84 +0.1 1,356,277 + BWI76_RS06450 0.48 +1.9 1,356,282 + BWI76_RS06450 0.50 +1.8 1,356,418 + -0.0 1,356,506 - +0.7 1,356,552 - +0.9 1,356,552 - -0.5 1,356,591 - +2.9 1,356,647 - BWI76_RS06455 0.11 +0.3 1,356,678 + BWI76_RS06455 0.13 +1.7 1,356,806 + BWI76_RS06455 0.21 +1.6 1,357,312 + BWI76_RS06455 0.51 +0.5 1,357,312 + BWI76_RS06455 0.51 +1.2 1,357,368 - BWI76_RS06455 0.54 +0.1 1,357,593 + BWI76_RS06455 0.68 +0.3 1,357,601 - BWI76_RS06455 0.68 +0.5 1,357,803 + BWI76_RS06455 0.80 +1.6 1,357,868 + BWI76_RS06455 0.84 -0.9 1,358,004 + +0.5 1,358,076 - +0.7 1,358,107 - -0.1 1,358,214 + BWI76_RS06460 0.10 +0.8 1,358,222 - BWI76_RS06460 0.11 +1.3 1,358,255 - BWI76_RS06460 0.15 -0.0 1,358,275 + BWI76_RS06460 0.18 +0.5 1,358,498 + BWI76_RS06460 0.45 +0.1 1,358,530 - BWI76_RS06460 0.49 -0.4 1,358,747 + BWI76_RS06460 0.75 +1.8
Or see this region's nucleotide sequence