Experiment: KLEB24 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06110 and BWI76_RS06115 overlap by 4 nucleotides BWI76_RS06115 and BWI76_RS06120 are separated by 255 nucleotides BWI76_RS06120 and BWI76_RS06125 are separated by 151 nucleotides
BWI76_RS06110: BWI76_RS06110 - hypothetical protein, at 1,284,849 to 1,285,955
_RS06110
BWI76_RS06115: BWI76_RS06115 - membrane protein, at 1,285,952 to 1,286,254
_RS06115
BWI76_RS06120: BWI76_RS06120 - membrane protein, at 1,286,510 to 1,286,713
_RS06120
BWI76_RS06125: BWI76_RS06125 - D-alanine--D-alanine ligase A, at 1,286,865 to 1,287,962
_RS06125
Position (kb)
1286
1287 Strain fitness (log2 ratio)
-2
-1
0
1 at 1285.944 kb on - strand at 1286.548 kb on - strand, within BWI76_RS06120 at 1286.553 kb on - strand, within BWI76_RS06120 at 1286.766 kb on + strand at 1286.965 kb on + strand at 1286.972 kb on + strand at 1287.054 kb on - strand, within BWI76_RS06125 at 1287.253 kb on + strand, within BWI76_RS06125 at 1287.364 kb on + strand, within BWI76_RS06125 at 1287.477 kb on + strand, within BWI76_RS06125 at 1287.490 kb on - strand, within BWI76_RS06125 at 1287.506 kb on + strand, within BWI76_RS06125 at 1287.657 kb on + strand, within BWI76_RS06125 at 1287.705 kb on + strand, within BWI76_RS06125
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB24 MOI 1 remove 1,285,944 - +1.1 1,286,548 - BWI76_RS06120 0.19 -1.3 1,286,553 - BWI76_RS06120 0.21 -2.0 1,286,766 + +0.0 1,286,965 + +0.0 1,286,972 + -1.3 1,287,054 - BWI76_RS06125 0.17 -0.1 1,287,253 + BWI76_RS06125 0.35 -0.3 1,287,364 + BWI76_RS06125 0.45 -1.5 1,287,477 + BWI76_RS06125 0.56 -0.1 1,287,490 - BWI76_RS06125 0.57 -0.5 1,287,506 + BWI76_RS06125 0.58 -0.4 1,287,657 + BWI76_RS06125 0.72 +0.0 1,287,705 + BWI76_RS06125 0.77 -0.7
Or see this region's nucleotide sequence