Experiment: KLEB24 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05725 and BWI76_RS05730 are separated by 453 nucleotides BWI76_RS05730 and BWI76_RS05735 are separated by 99 nucleotides BWI76_RS05735 and BWI76_RS05740 overlap by 14 nucleotides BWI76_RS05740 and BWI76_RS05745 are separated by 143 nucleotides
BWI76_RS05725: BWI76_RS05725 - beta-ketoacyl-[acyl-carrier-protein] synthase II, at 1,204,721 to 1,206,007
_RS05725
BWI76_RS05730: BWI76_RS05730 - hypothetical protein, at 1,206,461 to 1,207,207
_RS05730
BWI76_RS05735: BWI76_RS05735 - transcriptional regulator, at 1,207,307 to 1,207,675
_RS05735
BWI76_RS05740: BWI76_RS05740 - hypothetical protein, at 1,207,662 to 1,207,898
_RS05740
BWI76_RS05745: BWI76_RS05745 - hypothetical protein, at 1,208,042 to 1,209,280
_RS05745
Position (kb)
1206
1207
1208 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1205.533 kb on + strand, within BWI76_RS05725 at 1205.625 kb on - strand, within BWI76_RS05725 at 1205.625 kb on - strand, within BWI76_RS05725 at 1205.643 kb on - strand, within BWI76_RS05725 at 1205.684 kb on - strand, within BWI76_RS05725 at 1205.721 kb on - strand, within BWI76_RS05725 at 1205.723 kb on + strand, within BWI76_RS05725 at 1205.742 kb on - strand, within BWI76_RS05725 at 1205.775 kb on + strand, within BWI76_RS05725 at 1205.782 kb on + strand, within BWI76_RS05725 at 1205.809 kb on + strand, within BWI76_RS05725 at 1205.825 kb on + strand, within BWI76_RS05725 at 1205.838 kb on + strand, within BWI76_RS05725 at 1205.876 kb on - strand, within BWI76_RS05725 at 1205.885 kb on - strand at 1205.888 kb on + strand at 1205.888 kb on + strand at 1205.889 kb on + strand at 1205.897 kb on - strand at 1205.912 kb on - strand at 1205.916 kb on + strand at 1205.924 kb on + strand at 1205.924 kb on + strand at 1205.932 kb on - strand at 1205.932 kb on - strand at 1205.932 kb on - strand at 1205.932 kb on - strand at 1205.932 kb on - strand at 1205.932 kb on - strand at 1205.932 kb on - strand at 1205.949 kb on + strand at 1205.957 kb on - strand at 1205.957 kb on - strand at 1205.978 kb on + strand at 1206.021 kb on - strand at 1206.039 kb on + strand at 1206.047 kb on + strand at 1206.047 kb on - strand at 1206.049 kb on + strand at 1206.049 kb on + strand at 1206.049 kb on + strand at 1206.057 kb on - strand at 1206.092 kb on + strand at 1206.134 kb on + strand at 1206.145 kb on + strand at 1206.150 kb on - strand at 1206.152 kb on + strand at 1206.153 kb on - strand at 1206.170 kb on - strand at 1206.173 kb on - strand at 1206.180 kb on - strand at 1206.198 kb on + strand at 1206.208 kb on + strand at 1206.208 kb on + strand at 1206.208 kb on + strand at 1206.208 kb on + strand at 1206.208 kb on + strand at 1206.214 kb on - strand at 1206.214 kb on - strand at 1206.214 kb on - strand at 1206.216 kb on - strand at 1206.216 kb on - strand at 1206.216 kb on - strand at 1206.216 kb on - strand at 1206.216 kb on - strand at 1206.221 kb on - strand at 1206.270 kb on - strand at 1206.299 kb on - strand at 1206.303 kb on + strand at 1206.303 kb on + strand at 1206.311 kb on - strand at 1206.311 kb on - strand at 1206.311 kb on - strand at 1206.313 kb on + strand at 1206.329 kb on - strand at 1206.375 kb on - strand at 1206.402 kb on - strand at 1206.426 kb on + strand at 1206.426 kb on + strand at 1206.431 kb on + strand at 1206.434 kb on - strand at 1206.458 kb on + strand at 1206.458 kb on + strand at 1206.458 kb on + strand at 1206.458 kb on + strand at 1206.463 kb on - strand at 1206.466 kb on - strand at 1206.468 kb on + strand at 1206.471 kb on - strand at 1206.471 kb on - strand at 1206.518 kb on - strand at 1206.601 kb on + strand, within BWI76_RS05730 at 1206.601 kb on + strand, within BWI76_RS05730 at 1206.780 kb on + strand, within BWI76_RS05730 at 1206.780 kb on + strand, within BWI76_RS05730 at 1206.788 kb on - strand, within BWI76_RS05730 at 1206.893 kb on - strand, within BWI76_RS05730 at 1206.919 kb on + strand, within BWI76_RS05730 at 1206.919 kb on + strand, within BWI76_RS05730 at 1206.924 kb on + strand, within BWI76_RS05730 at 1206.995 kb on + strand, within BWI76_RS05730 at 1207.021 kb on + strand, within BWI76_RS05730 at 1207.021 kb on + strand, within BWI76_RS05730 at 1207.021 kb on + strand, within BWI76_RS05730 at 1207.029 kb on - strand, within BWI76_RS05730 at 1207.050 kb on + strand, within BWI76_RS05730 at 1207.050 kb on + strand, within BWI76_RS05730 at 1207.060 kb on - strand, within BWI76_RS05730 at 1207.101 kb on - strand, within BWI76_RS05730 at 1207.102 kb on + strand, within BWI76_RS05730 at 1207.110 kb on - strand, within BWI76_RS05730 at 1207.235 kb on + strand at 1207.241 kb on + strand at 1207.248 kb on + strand at 1207.256 kb on - strand at 1207.373 kb on + strand, within BWI76_RS05735 at 1207.449 kb on + strand, within BWI76_RS05735 at 1207.503 kb on + strand, within BWI76_RS05735 at 1207.503 kb on + strand, within BWI76_RS05735 at 1207.511 kb on - strand, within BWI76_RS05735 at 1207.551 kb on - strand, within BWI76_RS05735 at 1207.556 kb on + strand, within BWI76_RS05735 at 1207.566 kb on + strand, within BWI76_RS05735 at 1207.586 kb on + strand, within BWI76_RS05735 at 1207.588 kb on + strand, within BWI76_RS05735 at 1207.593 kb on + strand, within BWI76_RS05735 at 1207.598 kb on + strand, within BWI76_RS05735 at 1207.598 kb on + strand, within BWI76_RS05735 at 1207.598 kb on + strand, within BWI76_RS05735 at 1207.601 kb on - strand, within BWI76_RS05735 at 1207.603 kb on + strand, within BWI76_RS05735 at 1207.606 kb on - strand, within BWI76_RS05735 at 1207.618 kb on - strand, within BWI76_RS05735 at 1207.654 kb on + strand at 1207.659 kb on + strand at 1207.659 kb on + strand at 1207.667 kb on - strand at 1207.667 kb on - strand at 1207.667 kb on - strand at 1207.678 kb on - strand at 1207.701 kb on - strand, within BWI76_RS05740 at 1207.744 kb on + strand, within BWI76_RS05740 at 1207.768 kb on + strand, within BWI76_RS05740 at 1207.775 kb on + strand, within BWI76_RS05740 at 1207.775 kb on + strand, within BWI76_RS05740 at 1207.811 kb on - strand, within BWI76_RS05740 at 1207.839 kb on - strand, within BWI76_RS05740 at 1207.839 kb on - strand, within BWI76_RS05740 at 1208.008 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB24 MOI 1 remove 1,205,533 + BWI76_RS05725 0.63 +1.2 1,205,625 - BWI76_RS05725 0.70 -1.8 1,205,625 - BWI76_RS05725 0.70 -0.1 1,205,643 - BWI76_RS05725 0.72 -0.3 1,205,684 - BWI76_RS05725 0.75 +0.5 1,205,721 - BWI76_RS05725 0.78 -0.4 1,205,723 + BWI76_RS05725 0.78 +0.3 1,205,742 - BWI76_RS05725 0.79 +0.0 1,205,775 + BWI76_RS05725 0.82 -0.8 1,205,782 + BWI76_RS05725 0.82 +0.6 1,205,809 + BWI76_RS05725 0.85 -1.1 1,205,825 + BWI76_RS05725 0.86 -1.9 1,205,838 + BWI76_RS05725 0.87 -0.3 1,205,876 - BWI76_RS05725 0.90 -0.3 1,205,885 - -0.4 1,205,888 + -0.5 1,205,888 + -1.5 1,205,889 + -2.1 1,205,897 - -1.0 1,205,912 - -0.5 1,205,916 + -0.5 1,205,924 + +1.0 1,205,924 + -0.1 1,205,932 - +0.0 1,205,932 - +1.5 1,205,932 - -3.1 1,205,932 - +2.5 1,205,932 - -0.0 1,205,932 - -0.8 1,205,932 - -1.3 1,205,949 + -1.4 1,205,957 - +1.5 1,205,957 - -0.5 1,205,978 + +0.5 1,206,021 - -1.4 1,206,039 + -0.7 1,206,047 + +0.7 1,206,047 - -1.5 1,206,049 + -0.2 1,206,049 + +0.0 1,206,049 + -0.1 1,206,057 - -2.9 1,206,092 + -1.0 1,206,134 + -0.4 1,206,145 + -0.5 1,206,150 - -0.5 1,206,152 + -0.7 1,206,153 - +0.0 1,206,170 - -0.1 1,206,173 - +0.2 1,206,180 - -1.0 1,206,198 + -0.6 1,206,208 + -0.3 1,206,208 + -0.5 1,206,208 + -1.5 1,206,208 + +0.4 1,206,208 + -0.5 1,206,214 - +0.5 1,206,214 - +0.5 1,206,214 - -1.3 1,206,216 - -0.2 1,206,216 - -1.3 1,206,216 - -0.0 1,206,216 - -1.4 1,206,216 - -0.4 1,206,221 - -0.2 1,206,270 - +0.1 1,206,299 - +1.0 1,206,303 + -0.5 1,206,303 + -2.5 1,206,311 - -0.7 1,206,311 - -1.0 1,206,311 - +0.5 1,206,313 + +0.6 1,206,329 - -0.5 1,206,375 - +1.4 1,206,402 - +0.2 1,206,426 + -0.7 1,206,426 + +0.9 1,206,431 + -0.9 1,206,434 - -0.3 1,206,458 + -0.1 1,206,458 + -0.6 1,206,458 + -0.1 1,206,458 + -0.7 1,206,463 - -1.1 1,206,466 - -0.5 1,206,468 + -0.5 1,206,471 - +1.3 1,206,471 - -1.5 1,206,518 - +0.6 1,206,601 + BWI76_RS05730 0.19 -2.0 1,206,601 + BWI76_RS05730 0.19 -1.3 1,206,780 + BWI76_RS05730 0.43 -0.1 1,206,780 + BWI76_RS05730 0.43 +0.2 1,206,788 - BWI76_RS05730 0.44 -1.5 1,206,893 - BWI76_RS05730 0.58 +0.1 1,206,919 + BWI76_RS05730 0.61 -2.0 1,206,919 + BWI76_RS05730 0.61 -0.0 1,206,924 + BWI76_RS05730 0.62 -2.1 1,206,995 + BWI76_RS05730 0.71 -1.6 1,207,021 + BWI76_RS05730 0.75 -0.6 1,207,021 + BWI76_RS05730 0.75 -1.1 1,207,021 + BWI76_RS05730 0.75 -0.5 1,207,029 - BWI76_RS05730 0.76 -1.1 1,207,050 + BWI76_RS05730 0.79 -0.1 1,207,050 + BWI76_RS05730 0.79 -1.4 1,207,060 - BWI76_RS05730 0.80 +0.2 1,207,101 - BWI76_RS05730 0.86 -0.5 1,207,102 + BWI76_RS05730 0.86 +0.5 1,207,110 - BWI76_RS05730 0.87 -1.2 1,207,235 + -0.5 1,207,241 + -0.5 1,207,248 + -0.8 1,207,256 - -1.0 1,207,373 + BWI76_RS05735 0.18 -0.3 1,207,449 + BWI76_RS05735 0.38 +1.0 1,207,503 + BWI76_RS05735 0.53 +1.1 1,207,503 + BWI76_RS05735 0.53 -2.3 1,207,511 - BWI76_RS05735 0.55 -1.1 1,207,551 - BWI76_RS05735 0.66 -0.2 1,207,556 + BWI76_RS05735 0.67 -1.4 1,207,566 + BWI76_RS05735 0.70 -1.2 1,207,586 + BWI76_RS05735 0.76 +1.9 1,207,588 + BWI76_RS05735 0.76 -0.7 1,207,593 + BWI76_RS05735 0.78 -1.0 1,207,598 + BWI76_RS05735 0.79 -0.4 1,207,598 + BWI76_RS05735 0.79 -1.0 1,207,598 + BWI76_RS05735 0.79 -1.9 1,207,601 - BWI76_RS05735 0.80 -0.3 1,207,603 + BWI76_RS05735 0.80 -1.0 1,207,606 - BWI76_RS05735 0.81 -0.5 1,207,618 - BWI76_RS05735 0.84 +0.2 1,207,654 + -2.0 1,207,659 + -1.0 1,207,659 + -0.6 1,207,667 - +1.1 1,207,667 - +0.2 1,207,667 - -1.4 1,207,678 - -0.9 1,207,701 - BWI76_RS05740 0.16 -0.6 1,207,744 + BWI76_RS05740 0.35 -0.3 1,207,768 + BWI76_RS05740 0.45 +1.2 1,207,775 + BWI76_RS05740 0.48 +0.2 1,207,775 + BWI76_RS05740 0.48 -0.6 1,207,811 - BWI76_RS05740 0.63 -0.1 1,207,839 - BWI76_RS05740 0.75 -0.1 1,207,839 - BWI76_RS05740 0.75 -1.6 1,208,008 + -0.7
Or see this region's nucleotide sequence