Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS22965

Experiment: KLEB29 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS22955 and BWI76_RS22960 are separated by 353 nucleotidesBWI76_RS22960 and BWI76_RS22965 are separated by 198 nucleotidesBWI76_RS22965 and BWI76_RS22970 overlap by 1 nucleotidesBWI76_RS22970 and BWI76_RS22975 are separated by 139 nucleotides BWI76_RS22955: BWI76_RS22955 - transcriptional regulator GcvA, at 4,792,721 to 4,793,638 _RS22955 BWI76_RS22960: BWI76_RS22960 - YgdI/YgdR family lipoprotein, at 4,793,992 to 4,794,216 _RS22960 BWI76_RS22965: BWI76_RS22965 - cysteine sulfinate desulfinase, at 4,794,415 to 4,795,620 _RS22965 BWI76_RS22970: BWI76_RS22970 - cysteine desulfurase, sulfur acceptor subunit CsdE, at 4,795,620 to 4,796,060 _RS22970 BWI76_RS22975: BWI76_RS22975 - tRNA cyclic N6-threonylcarbamoyladenosine(37) synthase TcdA, at 4,796,200 to 4,797,009 _RS22975 Position (kb) 4794 4795 4796Strain fitness (log2 ratio) -2 -1 0 1at 4793.485 kb on + strand, within BWI76_RS22955at 4793.485 kb on + strand, within BWI76_RS22955at 4793.636 kb on - strandat 4793.832 kb on + strandat 4793.923 kb on - strandat 4794.029 kb on + strand, within BWI76_RS22960at 4794.168 kb on + strand, within BWI76_RS22960at 4794.176 kb on - strand, within BWI76_RS22960at 4794.266 kb on + strandat 4794.269 kb on - strandat 4794.422 kb on + strandat 4794.714 kb on - strand, within BWI76_RS22965at 4794.807 kb on + strand, within BWI76_RS22965at 4794.823 kb on - strand, within BWI76_RS22965at 4794.904 kb on - strand, within BWI76_RS22965at 4795.425 kb on + strand, within BWI76_RS22965at 4795.485 kb on - strand, within BWI76_RS22965at 4795.627 kb on + strandat 4795.683 kb on - strand, within BWI76_RS22970at 4796.063 kb on - strandat 4796.075 kb on + strandat 4796.151 kb on + strandat 4796.421 kb on - strand, within BWI76_RS22975at 4796.438 kb on + strand, within BWI76_RS22975

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB29 MOI 0.1
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4,793,485 + BWI76_RS22955 0.83 +0.4
4,793,485 + BWI76_RS22955 0.83 +1.1
4,793,636 - -1.2
4,793,832 + +0.5
4,793,923 - -0.6
4,794,029 + BWI76_RS22960 0.16 +0.6
4,794,168 + BWI76_RS22960 0.78 -0.1
4,794,176 - BWI76_RS22960 0.82 -0.5
4,794,266 + +0.6
4,794,269 - -1.6
4,794,422 + +0.5
4,794,714 - BWI76_RS22965 0.25 -0.6
4,794,807 + BWI76_RS22965 0.33 -0.3
4,794,823 - BWI76_RS22965 0.34 -2.1
4,794,904 - BWI76_RS22965 0.41 -0.5
4,795,425 + BWI76_RS22965 0.84 -0.3
4,795,485 - BWI76_RS22965 0.89 -0.9
4,795,627 + -1.3
4,795,683 - BWI76_RS22970 0.14 +0.9
4,796,063 - +0.4
4,796,075 + -0.5
4,796,151 + -0.5
4,796,421 - BWI76_RS22975 0.27 -0.5
4,796,438 + BWI76_RS22975 0.29 -0.1

Or see this region's nucleotide sequence