Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS17470

Experiment: KLEB29 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS17465 and BWI76_RS17470 are separated by 0 nucleotidesBWI76_RS17470 and BWI76_RS17475 are separated by 210 nucleotidesBWI76_RS17475 and BWI76_RS17480 are separated by 272 nucleotides BWI76_RS17465: BWI76_RS17465 - C4-dicarboxylic acid transporter DauA, at 3,621,017 to 3,622,696 _RS17465 BWI76_RS17470: BWI76_RS17470 - MerR family transcriptional regulator, at 3,622,697 to 3,623,743 _RS17470 BWI76_RS17475: BWI76_RS17475 - membrane protein, at 3,623,954 to 3,624,229 _RS17475 BWI76_RS17480: BWI76_RS17480 - aminoacyl-tRNA hydrolase, at 3,624,502 to 3,625,086 _RS17480 Position (kb) 3622 3623 3624Strain fitness (log2 ratio) -1 0 1at 3621.834 kb on - strand, within BWI76_RS17465at 3622.577 kb on - strandat 3622.577 kb on - strandat 3622.747 kb on + strandat 3622.773 kb on - strandat 3623.299 kb on + strand, within BWI76_RS17470at 3623.597 kb on - strand, within BWI76_RS17470at 3623.822 kb on + strandat 3624.178 kb on + strand, within BWI76_RS17475

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB29 MOI 0.1
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3,621,834 - BWI76_RS17465 0.49 +0.1
3,622,577 - +0.6
3,622,577 - -0.6
3,622,747 + +0.1
3,622,773 - +1.1
3,623,299 + BWI76_RS17470 0.57 -0.9
3,623,597 - BWI76_RS17470 0.86 -0.6
3,623,822 + -0.6
3,624,178 + BWI76_RS17475 0.81 +0.4

Or see this region's nucleotide sequence