Experiment: KLEB29 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14230 and BWI76_RS14235 are separated by 84 nucleotides BWI76_RS14235 and BWI76_RS14240 are separated by 43 nucleotides BWI76_RS14240 and BWI76_RS14245 are separated by 35 nucleotides
BWI76_RS14230: BWI76_RS14230 - flavin monoamine oxidase family protein, at 2,977,229 to 2,978,218
_RS14230
BWI76_RS14235: BWI76_RS14235 - alcohol dehydrogenase, at 2,978,303 to 2,979,327
_RS14235
BWI76_RS14240: BWI76_RS14240 - thiol reductase thioredoxin, at 2,979,371 to 2,979,694
_RS14240
BWI76_RS14245: BWI76_RS14245 - carboxymuconolactone decarboxylase family protein, at 2,979,730 to 2,980,251
_RS14245
Position (kb)
2978
2979
2980 Strain fitness (log2 ratio)
-2
-1
0
1 at 2977.381 kb on + strand, within BWI76_RS14230 at 2977.774 kb on + strand, within BWI76_RS14230 at 2977.774 kb on + strand, within BWI76_RS14230 at 2977.818 kb on - strand, within BWI76_RS14230 at 2977.994 kb on + strand, within BWI76_RS14230 at 2978.403 kb on - strand at 2978.449 kb on - strand, within BWI76_RS14235 at 2978.546 kb on - strand, within BWI76_RS14235 at 2978.552 kb on - strand, within BWI76_RS14235 at 2978.552 kb on - strand, within BWI76_RS14235 at 2978.702 kb on - strand, within BWI76_RS14235 at 2978.744 kb on + strand, within BWI76_RS14235 at 2978.892 kb on + strand, within BWI76_RS14235 at 2978.900 kb on - strand, within BWI76_RS14235 at 2978.940 kb on + strand, within BWI76_RS14235 at 2979.367 kb on + strand at 2979.468 kb on - strand, within BWI76_RS14240 at 2979.563 kb on - strand, within BWI76_RS14240 at 2979.626 kb on + strand, within BWI76_RS14240 at 2979.634 kb on - strand, within BWI76_RS14240 at 2979.743 kb on + strand at 2979.796 kb on + strand, within BWI76_RS14245 at 2979.926 kb on + strand, within BWI76_RS14245 at 2979.943 kb on - strand, within BWI76_RS14245 at 2979.990 kb on - strand, within BWI76_RS14245 at 2980.261 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB29 MOI 0.1 remove 2,977,381 + BWI76_RS14230 0.15 +0.5 2,977,774 + BWI76_RS14230 0.55 -0.1 2,977,774 + BWI76_RS14230 0.55 -0.2 2,977,818 - BWI76_RS14230 0.59 +0.5 2,977,994 + BWI76_RS14230 0.77 +0.0 2,978,403 - -0.0 2,978,449 - BWI76_RS14235 0.14 -1.2 2,978,546 - BWI76_RS14235 0.24 -0.3 2,978,552 - BWI76_RS14235 0.24 -2.0 2,978,552 - BWI76_RS14235 0.24 -0.4 2,978,702 - BWI76_RS14235 0.39 +0.3 2,978,744 + BWI76_RS14235 0.43 -0.5 2,978,892 + BWI76_RS14235 0.57 -0.1 2,978,900 - BWI76_RS14235 0.58 -2.0 2,978,940 + BWI76_RS14235 0.62 -0.1 2,979,367 + -0.7 2,979,468 - BWI76_RS14240 0.30 +0.2 2,979,563 - BWI76_RS14240 0.59 -0.4 2,979,626 + BWI76_RS14240 0.79 +0.8 2,979,634 - BWI76_RS14240 0.81 -1.3 2,979,743 + +0.2 2,979,796 + BWI76_RS14245 0.13 +0.6 2,979,926 + BWI76_RS14245 0.38 -2.0 2,979,943 - BWI76_RS14245 0.41 +0.2 2,979,990 - BWI76_RS14245 0.50 -1.0 2,980,261 + +0.2
Or see this region's nucleotide sequence