Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14060

Experiment: KLEB29 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14055 and BWI76_RS14060 are separated by 168 nucleotidesBWI76_RS14060 and BWI76_RS14065 are separated by 338 nucleotidesBWI76_RS14065 and BWI76_RS14070 are separated by 5 nucleotides BWI76_RS14055: BWI76_RS14055 - LacI family transcriptional regulator, at 2,937,277 to 2,938,311 _RS14055 BWI76_RS14060: BWI76_RS14060 - secretory lipase, at 2,938,480 to 2,939,622 _RS14060 BWI76_RS14065: BWI76_RS14065 - hypothetical protein, at 2,939,961 to 2,940,386 _RS14065 BWI76_RS14070: BWI76_RS14070 - ECF subfamily RNA polymerase sigma-24 subunit, at 2,940,392 to 2,940,952 _RS14070 Position (kb) 2938 2939 2940Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2937.617 kb on - strand, within BWI76_RS14055at 2937.626 kb on + strand, within BWI76_RS14055at 2937.671 kb on - strandat 2937.853 kb on + strand, within BWI76_RS14055at 2937.856 kb on + strand, within BWI76_RS14055at 2937.863 kb on - strand, within BWI76_RS14055at 2937.888 kb on - strand, within BWI76_RS14055at 2938.249 kb on - strandat 2938.421 kb on + strandat 2938.452 kb on - strandat 2938.492 kb on - strandat 2938.525 kb on - strandat 2938.801 kb on - strand, within BWI76_RS14060at 2938.858 kb on - strand, within BWI76_RS14060at 2939.004 kb on + strand, within BWI76_RS14060at 2939.229 kb on + strand, within BWI76_RS14060at 2939.381 kb on - strand, within BWI76_RS14060at 2939.675 kb on - strandat 2939.823 kb on - strandat 2939.923 kb on - strandat 2939.923 kb on - strandat 2939.925 kb on + strandat 2939.933 kb on - strandat 2940.146 kb on - strand, within BWI76_RS14065at 2940.253 kb on + strand, within BWI76_RS14065at 2940.261 kb on - strand, within BWI76_RS14065

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB29 MOI 0.1
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2,937,617 - BWI76_RS14055 0.33 -0.7
2,937,626 + BWI76_RS14055 0.34 -0.8
2,937,671 - +2.8
2,937,853 + BWI76_RS14055 0.56 +1.3
2,937,856 + BWI76_RS14055 0.56 +0.1
2,937,863 - BWI76_RS14055 0.57 -0.8
2,937,888 - BWI76_RS14055 0.59 +1.3
2,938,249 - +0.9
2,938,421 + +0.8
2,938,452 - +0.1
2,938,492 - +0.8
2,938,525 - -2.3
2,938,801 - BWI76_RS14060 0.28 -2.1
2,938,858 - BWI76_RS14060 0.33 -0.1
2,939,004 + BWI76_RS14060 0.46 -0.9
2,939,229 + BWI76_RS14060 0.66 -0.5
2,939,381 - BWI76_RS14060 0.79 -0.6
2,939,675 - +0.0
2,939,823 - +0.2
2,939,923 - +0.2
2,939,923 - -0.8
2,939,925 + +1.2
2,939,933 - -0.3
2,940,146 - BWI76_RS14065 0.43 -1.7
2,940,253 + BWI76_RS14065 0.69 +0.7
2,940,261 - BWI76_RS14065 0.70 -2.3

Or see this region's nucleotide sequence