Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14020

Experiment: KLEB29 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14015 and BWI76_RS14020 are separated by 18 nucleotidesBWI76_RS14020 and BWI76_RS14025 are separated by 307 nucleotides BWI76_RS14015: BWI76_RS14015 - L-2,4-diaminobutyrate decarboxylase, at 2,928,356 to 2,929,828 _RS14015 BWI76_RS14020: BWI76_RS14020 - aspartate aminotransferase family protein, at 2,929,847 to 2,931,232 _RS14020 BWI76_RS14025: BWI76_RS14025 - NMT1/THI5 like domain-containing protein, at 2,931,540 to 2,932,475 _RS14025 Position (kb) 2929 2930 2931 2932Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2929.084 kb on + strand, within BWI76_RS14015at 2929.119 kb on + strand, within BWI76_RS14015at 2929.127 kb on - strand, within BWI76_RS14015at 2929.734 kb on - strandat 2930.517 kb on + strand, within BWI76_RS14020at 2930.675 kb on - strand, within BWI76_RS14020at 2931.084 kb on + strand, within BWI76_RS14020at 2931.084 kb on - strand, within BWI76_RS14020at 2931.084 kb on - strand, within BWI76_RS14020at 2931.152 kb on - strandat 2931.185 kb on + strandat 2931.185 kb on + strandat 2931.239 kb on + strandat 2931.239 kb on + strandat 2931.241 kb on + strandat 2931.245 kb on - strandat 2931.247 kb on - strandat 2931.247 kb on - strandat 2931.247 kb on - strandat 2931.249 kb on + strandat 2931.249 kb on + strandat 2931.249 kb on + strandat 2931.257 kb on - strandat 2931.257 kb on - strandat 2931.260 kb on - strandat 2931.265 kb on - strandat 2931.265 kb on - strandat 2931.311 kb on - strandat 2931.320 kb on + strandat 2931.320 kb on + strandat 2931.328 kb on - strandat 2931.377 kb on + strandat 2931.377 kb on + strandat 2931.386 kb on - strandat 2931.533 kb on + strandat 2931.538 kb on + strandat 2931.546 kb on - strandat 2931.546 kb on - strandat 2931.546 kb on - strandat 2931.546 kb on - strandat 2931.588 kb on - strandat 2931.589 kb on + strandat 2931.600 kb on + strandat 2931.601 kb on + strandat 2931.619 kb on - strandat 2931.620 kb on - strandat 2931.639 kb on - strand, within BWI76_RS14025at 2931.665 kb on + strand, within BWI76_RS14025at 2931.672 kb on + strand, within BWI76_RS14025at 2931.703 kb on + strand, within BWI76_RS14025at 2931.703 kb on + strand, within BWI76_RS14025at 2931.711 kb on - strand, within BWI76_RS14025at 2931.734 kb on + strand, within BWI76_RS14025at 2931.772 kb on - strand, within BWI76_RS14025at 2931.898 kb on + strand, within BWI76_RS14025at 2932.013 kb on - strand, within BWI76_RS14025at 2932.197 kb on + strand, within BWI76_RS14025

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB29 MOI 0.1
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2,929,084 + BWI76_RS14015 0.49 +3.5
2,929,119 + BWI76_RS14015 0.52 -0.4
2,929,127 - BWI76_RS14015 0.52 -0.1
2,929,734 - -0.2
2,930,517 + BWI76_RS14020 0.48 +2.3
2,930,675 - BWI76_RS14020 0.60 -0.6
2,931,084 + BWI76_RS14020 0.89 -1.0
2,931,084 - BWI76_RS14020 0.89 +0.0
2,931,084 - BWI76_RS14020 0.89 -0.6
2,931,152 - +1.0
2,931,185 + +0.1
2,931,185 + +0.5
2,931,239 + +0.1
2,931,239 + +0.3
2,931,241 + +1.8
2,931,245 - -0.2
2,931,247 - +0.1
2,931,247 - -0.7
2,931,247 - -0.7
2,931,249 + -0.1
2,931,249 + -2.1
2,931,249 + -0.8
2,931,257 - +0.1
2,931,257 - -0.3
2,931,260 - -0.0
2,931,265 - -0.6
2,931,265 - -0.3
2,931,311 - -0.2
2,931,320 + +0.8
2,931,320 + -0.8
2,931,328 - -0.3
2,931,377 + -1.3
2,931,377 + -0.2
2,931,386 - +2.1
2,931,533 + -0.3
2,931,538 + -0.0
2,931,546 - -0.2
2,931,546 - -0.8
2,931,546 - -0.6
2,931,546 - -0.3
2,931,588 - +0.8
2,931,589 + +1.1
2,931,600 + -0.4
2,931,601 + -1.3
2,931,619 - +0.5
2,931,620 - -0.4
2,931,639 - BWI76_RS14025 0.11 -0.5
2,931,665 + BWI76_RS14025 0.13 -0.1
2,931,672 + BWI76_RS14025 0.14 -0.9
2,931,703 + BWI76_RS14025 0.17 +0.5
2,931,703 + BWI76_RS14025 0.17 +1.6
2,931,711 - BWI76_RS14025 0.18 +0.4
2,931,734 + BWI76_RS14025 0.21 -0.6
2,931,772 - BWI76_RS14025 0.25 -0.1
2,931,898 + BWI76_RS14025 0.38 -0.2
2,932,013 - BWI76_RS14025 0.51 +0.4
2,932,197 + BWI76_RS14025 0.70 +0.0

Or see this region's nucleotide sequence