Experiment: KLEB29 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11900 and BWI76_RS11905 overlap by 4 nucleotides BWI76_RS11905 and BWI76_RS11910 overlap by 6 nucleotides BWI76_RS11910 and BWI76_RS11915 overlap by 1 nucleotides BWI76_RS11915 and BWI76_RS11920 are separated by 56 nucleotides BWI76_RS11920 and BWI76_RS11925 are separated by 410 nucleotides
BWI76_RS11900: BWI76_RS11900 - cob(I)yrinic acid a,c-diamide adenosyltransferase, at 2,505,262 to 2,505,852
_RS11900
BWI76_RS11905: BWI76_RS11905 - NAD(P)-dependent oxidoreductase, at 2,505,849 to 2,506,610
_RS11905
BWI76_RS11910: BWI76_RS11910 - hypothetical protein, at 2,506,605 to 2,506,820
_RS11910
BWI76_RS11915: BWI76_RS11915 - protease SohB, at 2,506,820 to 2,507,911
_RS11915
BWI76_RS11920: BWI76_RS11920 - hypothetical protein, at 2,507,968 to 2,508,219
_RS11920
BWI76_RS11925: BWI76_RS11925 - DNA topoisomerase I, at 2,508,630 to 2,511,227
_RS11925
Position (kb)
2506
2507
2508 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2505.820 kb on + strand at 2506.228 kb on - strand, within BWI76_RS11905 at 2506.821 kb on + strand at 2507.022 kb on + strand, within BWI76_RS11915 at 2507.202 kb on + strand, within BWI76_RS11915 at 2507.405 kb on + strand, within BWI76_RS11915 at 2507.666 kb on - strand, within BWI76_RS11915 at 2507.885 kb on - strand at 2507.946 kb on - strand at 2508.058 kb on - strand, within BWI76_RS11920 at 2508.082 kb on + strand, within BWI76_RS11920 at 2508.106 kb on - strand, within BWI76_RS11920 at 2508.106 kb on - strand, within BWI76_RS11920 at 2508.255 kb on - strand at 2508.383 kb on + strand at 2508.521 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB29 MOI 0.1 remove 2,505,820 + -2.2 2,506,228 - BWI76_RS11905 0.50 -0.0 2,506,821 + +0.1 2,507,022 + BWI76_RS11915 0.18 -0.6 2,507,202 + BWI76_RS11915 0.35 -0.3 2,507,405 + BWI76_RS11915 0.54 -0.8 2,507,666 - BWI76_RS11915 0.77 -0.7 2,507,885 - -1.8 2,507,946 - -1.3 2,508,058 - BWI76_RS11920 0.36 +2.7 2,508,082 + BWI76_RS11920 0.45 +1.4 2,508,106 - BWI76_RS11920 0.55 -0.3 2,508,106 - BWI76_RS11920 0.55 -0.9 2,508,255 - -0.5 2,508,383 + -0.3 2,508,521 + +0.5
Or see this region's nucleotide sequence