Experiment: KLEB29 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08800 and BWI76_RS08805 are separated by 111 nucleotides BWI76_RS08805 and BWI76_RS08810 are separated by 28 nucleotides BWI76_RS08810 and BWI76_RS08815 are separated by 111 nucleotides
BWI76_RS08800: BWI76_RS08800 - phosphoethanolamine transferase EptA, at 1,855,514 to 1,857,157
_RS08800
BWI76_RS08805: BWI76_RS08805 - gluconeogenesis factor, at 1,857,269 to 1,858,174
_RS08805
BWI76_RS08810: BWI76_RS08810 - hypothetical protein, at 1,858,203 to 1,858,397
_RS08810
BWI76_RS08815: BWI76_RS08815 - cyclic pyranopterin phosphate synthase, at 1,858,509 to 1,859,508
_RS08815
Position (kb)
1857
1858
1859 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1856.866 kb on + strand, within BWI76_RS08800 at 1857.043 kb on + strand at 1857.130 kb on - strand at 1857.170 kb on + strand at 1857.267 kb on + strand at 1857.274 kb on - strand at 1857.288 kb on + strand at 1857.296 kb on - strand at 1857.313 kb on - strand at 1857.347 kb on + strand at 1857.491 kb on + strand, within BWI76_RS08805 at 1857.550 kb on - strand, within BWI76_RS08805 at 1857.725 kb on + strand, within BWI76_RS08805 at 1857.978 kb on - strand, within BWI76_RS08805 at 1858.072 kb on + strand, within BWI76_RS08805 at 1858.214 kb on + strand at 1858.397 kb on + strand at 1858.397 kb on + strand at 1858.404 kb on - strand at 1858.505 kb on + strand at 1858.513 kb on + strand at 1858.833 kb on + strand, within BWI76_RS08815 at 1859.001 kb on - strand, within BWI76_RS08815 at 1859.011 kb on + strand, within BWI76_RS08815 at 1859.062 kb on + strand, within BWI76_RS08815
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB29 MOI 0.1 remove 1,856,866 + BWI76_RS08800 0.82 +0.0 1,857,043 + +2.9 1,857,130 - -0.2 1,857,170 + +0.4 1,857,267 + -0.5 1,857,274 - -0.3 1,857,288 + -0.7 1,857,296 - +0.0 1,857,313 - +0.5 1,857,347 + +0.1 1,857,491 + BWI76_RS08805 0.25 -0.2 1,857,550 - BWI76_RS08805 0.31 -1.1 1,857,725 + BWI76_RS08805 0.50 -0.5 1,857,978 - BWI76_RS08805 0.78 -1.0 1,858,072 + BWI76_RS08805 0.89 -0.7 1,858,214 + -0.4 1,858,397 + +0.1 1,858,397 + +0.5 1,858,404 - -2.5 1,858,505 + +0.1 1,858,513 + +0.7 1,858,833 + BWI76_RS08815 0.32 +1.6 1,859,001 - BWI76_RS08815 0.49 -0.2 1,859,011 + BWI76_RS08815 0.50 +0.7 1,859,062 + BWI76_RS08815 0.55 -0.5
Or see this region's nucleotide sequence