Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS26900

Experiment: KLEB29 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS26885 and BWI76_RS26890 are separated by 98 nucleotidesBWI76_RS26890 and BWI76_RS26895 are separated by 429 nucleotidesBWI76_RS26895 and BWI76_RS26900 are separated by 89 nucleotidesBWI76_RS26900 and BWI76_RS26905 are separated by 241 nucleotides BWI76_RS26885: BWI76_RS26885 - ABC transporter substrate-binding protein, at 5,577,554 to 5,579,161 _RS26885 BWI76_RS26890: BWI76_RS26890 - hypothetical protein, at 5,579,260 to 5,579,502 _RS26890 BWI76_RS26895: BWI76_RS26895 - tRNA-Pro, at 5,579,932 to 5,580,008 _RS26895 BWI76_RS26900: BWI76_RS26900 - phosphoethanolamine transferase, at 5,580,098 to 5,581,786 _RS26900 BWI76_RS26905: BWI76_RS26905 - oxalate/formate antiport family MFS transporter, at 5,582,028 to 5,583,230 _RS26905 Position (kb) 5580 5581 5582Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5 6at 5579.100 kb on - strandat 5579.121 kb on - strandat 5579.121 kb on - strandat 5579.122 kb on - strandat 5579.124 kb on + strandat 5579.132 kb on - strandat 5579.193 kb on + strandat 5579.230 kb on + strandat 5579.238 kb on - strandat 5579.247 kb on + strandat 5579.257 kb on - strandat 5579.267 kb on - strandat 5579.270 kb on - strandat 5579.303 kb on - strand, within BWI76_RS26890at 5579.476 kb on - strand, within BWI76_RS26890at 5579.715 kb on + strandat 5579.715 kb on + strandat 5579.715 kb on + strandat 5579.715 kb on + strandat 5579.723 kb on - strandat 5579.725 kb on - strandat 5579.753 kb on + strandat 5579.867 kb on + strandat 5580.174 kb on - strandat 5580.183 kb on - strandat 5580.193 kb on - strandat 5580.193 kb on - strandat 5580.272 kb on - strand, within BWI76_RS26900at 5580.497 kb on - strand, within BWI76_RS26900at 5580.550 kb on + strand, within BWI76_RS26900at 5580.550 kb on + strand, within BWI76_RS26900at 5580.550 kb on + strand, within BWI76_RS26900at 5580.558 kb on - strand, within BWI76_RS26900at 5580.577 kb on - strand, within BWI76_RS26900at 5580.708 kb on + strand, within BWI76_RS26900at 5580.708 kb on + strand, within BWI76_RS26900at 5580.758 kb on + strand, within BWI76_RS26900at 5580.780 kb on + strand, within BWI76_RS26900at 5580.788 kb on - strand, within BWI76_RS26900at 5580.788 kb on - strand, within BWI76_RS26900at 5580.788 kb on - strand, within BWI76_RS26900at 5580.825 kb on - strand, within BWI76_RS26900at 5580.872 kb on - strand, within BWI76_RS26900at 5580.888 kb on - strand, within BWI76_RS26900at 5580.890 kb on - strand, within BWI76_RS26900at 5581.057 kb on - strand, within BWI76_RS26900at 5581.153 kb on - strand, within BWI76_RS26900at 5581.248 kb on + strand, within BWI76_RS26900at 5581.356 kb on - strand, within BWI76_RS26900at 5581.397 kb on + strand, within BWI76_RS26900at 5581.397 kb on + strand, within BWI76_RS26900at 5581.405 kb on - strand, within BWI76_RS26900at 5581.535 kb on + strand, within BWI76_RS26900at 5581.563 kb on + strand, within BWI76_RS26900at 5581.623 kb on + strandat 5581.670 kb on + strandat 5581.680 kb on - strandat 5581.684 kb on + strandat 5581.684 kb on - strandat 5581.760 kb on - strandat 5581.846 kb on + strandat 5581.846 kb on + strandat 5581.881 kb on + strandat 5581.881 kb on + strandat 5581.881 kb on + strandat 5581.889 kb on - strandat 5581.889 kb on - strandat 5581.906 kb on - strandat 5581.908 kb on - strandat 5581.938 kb on - strandat 5582.092 kb on - strandat 5582.164 kb on + strand, within BWI76_RS26905at 5582.498 kb on - strand, within BWI76_RS26905at 5582.502 kb on + strand, within BWI76_RS26905at 5582.542 kb on - strand, within BWI76_RS26905at 5582.590 kb on + strand, within BWI76_RS26905at 5582.591 kb on - strand, within BWI76_RS26905

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB29 MOI 1
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5,579,100 - +0.2
5,579,121 - +0.3
5,579,121 - +0.2
5,579,122 - -0.9
5,579,124 + +1.1
5,579,132 - -0.9
5,579,193 + +0.7
5,579,230 + -3.5
5,579,238 - +0.2
5,579,247 + -0.1
5,579,257 - -1.0
5,579,267 - +0.7
5,579,270 - -0.7
5,579,303 - BWI76_RS26890 0.18 +0.6
5,579,476 - BWI76_RS26890 0.89 -0.1
5,579,715 + -0.9
5,579,715 + +0.9
5,579,715 + -0.3
5,579,715 + -1.5
5,579,723 - +0.4
5,579,725 - +0.7
5,579,753 + -0.0
5,579,867 + +0.2
5,580,174 - +1.6
5,580,183 - +0.3
5,580,193 - +0.5
5,580,193 - +1.0
5,580,272 - BWI76_RS26900 0.10 +1.8
5,580,497 - BWI76_RS26900 0.24 +0.3
5,580,550 + BWI76_RS26900 0.27 +0.7
5,580,550 + BWI76_RS26900 0.27 +1.0
5,580,550 + BWI76_RS26900 0.27 +1.1
5,580,558 - BWI76_RS26900 0.27 +0.3
5,580,577 - BWI76_RS26900 0.28 +2.0
5,580,708 + BWI76_RS26900 0.36 +1.0
5,580,708 + BWI76_RS26900 0.36 +1.2
5,580,758 + BWI76_RS26900 0.39 +6.0
5,580,780 + BWI76_RS26900 0.40 +1.2
5,580,788 - BWI76_RS26900 0.41 +1.7
5,580,788 - BWI76_RS26900 0.41 +2.5
5,580,788 - BWI76_RS26900 0.41 +1.6
5,580,825 - BWI76_RS26900 0.43 +2.1
5,580,872 - BWI76_RS26900 0.46 +2.8
5,580,888 - BWI76_RS26900 0.47 +0.5
5,580,890 - BWI76_RS26900 0.47 +1.2
5,581,057 - BWI76_RS26900 0.57 +0.8
5,581,153 - BWI76_RS26900 0.62 +3.2
5,581,248 + BWI76_RS26900 0.68 -0.5
5,581,356 - BWI76_RS26900 0.74 +1.7
5,581,397 + BWI76_RS26900 0.77 +1.7
5,581,397 + BWI76_RS26900 0.77 +1.5
5,581,405 - BWI76_RS26900 0.77 +1.4
5,581,535 + BWI76_RS26900 0.85 +1.3
5,581,563 + BWI76_RS26900 0.87 +0.6
5,581,623 + +1.0
5,581,670 + +0.6
5,581,680 - +1.1
5,581,684 + +1.7
5,581,684 - +1.4
5,581,760 - +1.6
5,581,846 + +0.7
5,581,846 + +1.8
5,581,881 + +1.5
5,581,881 + +2.4
5,581,881 + +1.4
5,581,889 - -1.2
5,581,889 - -0.1
5,581,906 - -0.4
5,581,908 - +1.0
5,581,938 - +1.7
5,582,092 - -0.0
5,582,164 + BWI76_RS26905 0.11 -0.4
5,582,498 - BWI76_RS26905 0.39 +2.2
5,582,502 + BWI76_RS26905 0.39 -0.5
5,582,542 - BWI76_RS26905 0.43 -0.3
5,582,590 + BWI76_RS26905 0.47 +0.4
5,582,591 - BWI76_RS26905 0.47 -2.3

Or see this region's nucleotide sequence