Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25085

Experiment: KLEB29 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25080 and BWI76_RS25085 overlap by 1 nucleotidesBWI76_RS25085 and BWI76_RS25090 are separated by 91 nucleotides BWI76_RS25080: BWI76_RS25080 - tRNA pseudouridine(55) synthase, at 5,221,243 to 5,222,187 _RS25080 BWI76_RS25085: BWI76_RS25085 - ribosome-binding factor A, at 5,222,187 to 5,222,588 _RS25085 BWI76_RS25090: BWI76_RS25090 - translation initiation factor IF-2, at 5,222,680 to 5,225,370 _RS25090 Position (kb) 5222 5223Strain fitness (log2 ratio) -1 0 1at 5221.250 kb on + strandat 5221.407 kb on - strand, within BWI76_RS25080at 5221.453 kb on + strand, within BWI76_RS25080at 5221.520 kb on + strand, within BWI76_RS25080at 5221.746 kb on - strand, within BWI76_RS25080at 5221.852 kb on + strand, within BWI76_RS25080

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB29 MOI 1
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5,221,250 + +0.6
5,221,407 - BWI76_RS25080 0.17 -0.0
5,221,453 + BWI76_RS25080 0.22 -1.0
5,221,520 + BWI76_RS25080 0.29 +1.3
5,221,746 - BWI76_RS25080 0.53 +1.5
5,221,852 + BWI76_RS25080 0.64 +0.2

Or see this region's nucleotide sequence