Experiment: KLEB29 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25065 and BWI76_RS25070 overlap by 42 nucleotides BWI76_RS25070 and BWI76_RS25075 are separated by 71 nucleotides BWI76_RS25075 and BWI76_RS25080 are separated by 147 nucleotides
BWI76_RS25065: BWI76_RS25065 - polyribonucleotide nucleotidyltransferase, at 5,218,446 to 5,220,584
_RS25065
BWI76_RS25070: BWI76_RS25070 - hypothetical protein, at 5,220,543 to 5,220,754
_RS25070
BWI76_RS25075: BWI76_RS25075 - 30S ribosomal protein S15, at 5,220,826 to 5,221,095
_RS25075
BWI76_RS25080: BWI76_RS25080 - tRNA pseudouridine(55) synthase, at 5,221,243 to 5,222,187
_RS25080
Position (kb) 5220
5221
5222 Strain fitness (log2 ratio) -1
0
1
2 at 5220.072 kb on - strand, within BWI76_RS25065 at 5220.091 kb on + strand, within BWI76_RS25065 at 5220.430 kb on + strand at 5220.472 kb on + strand at 5220.681 kb on - strand, within BWI76_RS25070 at 5220.709 kb on - strand at 5221.250 kb on + strand at 5221.407 kb on - strand, within BWI76_RS25080 at 5221.453 kb on + strand, within BWI76_RS25080 at 5221.520 kb on + strand, within BWI76_RS25080 at 5221.746 kb on - strand, within BWI76_RS25080 at 5221.852 kb on + strand, within BWI76_RS25080
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB29 MOI 1 remove 5,220,072 - BWI76_RS25065 0.76 +1.7 5,220,091 + BWI76_RS25065 0.77 +2.3 5,220,430 + +1.8 5,220,472 + +1.3 5,220,681 - BWI76_RS25070 0.65 +0.4 5,220,709 - +0.6 5,221,250 + +0.6 5,221,407 - BWI76_RS25080 0.17 -0.0 5,221,453 + BWI76_RS25080 0.22 -1.0 5,221,520 + BWI76_RS25080 0.29 +1.3 5,221,746 - BWI76_RS25080 0.53 +1.5 5,221,852 + BWI76_RS25080 0.64 +0.2
Or see this region's nucleotide sequence