Experiment: KLEB29 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24050 and BWI76_RS24055 are separated by 379 nucleotides BWI76_RS24055 and BWI76_RS24060 are separated by 75 nucleotides BWI76_RS24060 and BWI76_RS24065 are separated by 94 nucleotides
BWI76_RS24050: BWI76_RS24050 - methionine adenosyltransferase, at 5,018,179 to 5,019,333
_RS24050
BWI76_RS24055: BWI76_RS24055 - MFS transporter, at 5,019,713 to 5,021,107
_RS24055
BWI76_RS24060: BWI76_RS24060 - SprT family protein, at 5,021,183 to 5,021,683
_RS24060
BWI76_RS24065: BWI76_RS24065 - deoxyribonuclease I, at 5,021,778 to 5,022,485
_RS24065
Position (kb) 5019
5020
5021
5022 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 5019.413 kb on + strand at 5019.421 kb on - strand at 5019.484 kb on + strand at 5019.717 kb on - strand at 5019.835 kb on - strand at 5019.835 kb on - strand at 5019.888 kb on + strand, within BWI76_RS24055 at 5019.922 kb on + strand, within BWI76_RS24055 at 5020.376 kb on - strand, within BWI76_RS24055 at 5020.410 kb on + strand, within BWI76_RS24055 at 5020.674 kb on + strand, within BWI76_RS24055 at 5020.764 kb on - strand, within BWI76_RS24055 at 5020.807 kb on - strand, within BWI76_RS24055 at 5021.081 kb on - strand at 5021.275 kb on + strand, within BWI76_RS24060 at 5021.283 kb on - strand, within BWI76_RS24060 at 5021.769 kb on + strand at 5021.875 kb on - strand, within BWI76_RS24065
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB29 MOI 1 remove 5,019,413 + +1.4 5,019,421 - -1.0 5,019,484 + +3.0 5,019,717 - -2.9 5,019,835 - -3.2 5,019,835 - -1.6 5,019,888 + BWI76_RS24055 0.13 -1.9 5,019,922 + BWI76_RS24055 0.15 -3.8 5,020,376 - BWI76_RS24055 0.48 -2.4 5,020,410 + BWI76_RS24055 0.50 -4.3 5,020,674 + BWI76_RS24055 0.69 -0.5 5,020,764 - BWI76_RS24055 0.75 -0.1 5,020,807 - BWI76_RS24055 0.78 +0.3 5,021,081 - -1.9 5,021,275 + BWI76_RS24060 0.18 +0.6 5,021,283 - BWI76_RS24060 0.20 +0.2 5,021,769 + -0.5 5,021,875 - BWI76_RS24065 0.14 -1.1
Or see this region's nucleotide sequence