Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS22815

Experiment: KLEB29 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS22810 and BWI76_RS22815 are separated by 124 nucleotidesBWI76_RS22815 and BWI76_RS22820 are separated by 40 nucleotides BWI76_RS22810: BWI76_RS22810 - 23S rRNA (uracil(1939)-C(5))-methyltransferase, at 4,757,086 to 4,758,429 _RS22810 BWI76_RS22815: BWI76_RS22815 - hybrid sensor histidine kinase/response regulator, at 4,758,554 to 4,761,307 _RS22815 BWI76_RS22820: BWI76_RS22820 - glycerate kinase, at 4,761,348 to 4,762,487 _RS22820 Position (kb) 4758 4759 4760 4761 4762Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 4757.637 kb on + strand, within BWI76_RS22810at 4757.673 kb on + strand, within BWI76_RS22810at 4757.689 kb on + strand, within BWI76_RS22810at 4757.945 kb on - strand, within BWI76_RS22810at 4758.076 kb on - strand, within BWI76_RS22810at 4758.227 kb on + strand, within BWI76_RS22810at 4758.227 kb on + strand, within BWI76_RS22810at 4758.227 kb on + strand, within BWI76_RS22810at 4758.235 kb on - strand, within BWI76_RS22810at 4758.377 kb on + strandat 4758.377 kb on + strandat 4758.444 kb on - strandat 4758.490 kb on - strandat 4758.490 kb on - strandat 4758.520 kb on - strandat 4758.561 kb on + strandat 4758.569 kb on - strandat 4758.608 kb on + strandat 4758.672 kb on + strandat 4758.704 kb on - strandat 4758.891 kb on - strand, within BWI76_RS22815at 4758.959 kb on - strand, within BWI76_RS22815at 4759.116 kb on - strand, within BWI76_RS22815at 4759.214 kb on + strand, within BWI76_RS22815at 4759.234 kb on - strand, within BWI76_RS22815at 4759.482 kb on - strand, within BWI76_RS22815at 4759.595 kb on + strand, within BWI76_RS22815at 4759.670 kb on - strand, within BWI76_RS22815at 4759.685 kb on - strand, within BWI76_RS22815at 4759.708 kb on - strand, within BWI76_RS22815at 4759.713 kb on + strand, within BWI76_RS22815at 4760.066 kb on - strand, within BWI76_RS22815at 4760.092 kb on - strand, within BWI76_RS22815at 4760.103 kb on + strand, within BWI76_RS22815at 4760.126 kb on + strand, within BWI76_RS22815at 4760.134 kb on - strand, within BWI76_RS22815at 4760.149 kb on + strand, within BWI76_RS22815at 4760.168 kb on - strand, within BWI76_RS22815at 4760.330 kb on - strand, within BWI76_RS22815at 4760.412 kb on - strand, within BWI76_RS22815at 4760.606 kb on + strand, within BWI76_RS22815at 4760.646 kb on + strand, within BWI76_RS22815at 4761.020 kb on - strand, within BWI76_RS22815at 4761.096 kb on - strandat 4761.103 kb on + strandat 4761.328 kb on - strandat 4761.435 kb on - strandat 4761.571 kb on + strand, within BWI76_RS22820at 4761.781 kb on - strand, within BWI76_RS22820at 4761.943 kb on + strand, within BWI76_RS22820at 4761.978 kb on + strand, within BWI76_RS22820at 4761.978 kb on + strand, within BWI76_RS22820at 4761.986 kb on - strand, within BWI76_RS22820

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB29 MOI 1
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4,757,637 + BWI76_RS22810 0.41 -0.4
4,757,673 + BWI76_RS22810 0.44 -0.2
4,757,689 + BWI76_RS22810 0.45 -1.1
4,757,945 - BWI76_RS22810 0.64 +0.8
4,758,076 - BWI76_RS22810 0.74 +0.7
4,758,227 + BWI76_RS22810 0.85 +0.1
4,758,227 + BWI76_RS22810 0.85 -2.7
4,758,227 + BWI76_RS22810 0.85 -0.4
4,758,235 - BWI76_RS22810 0.85 -0.6
4,758,377 + -0.6
4,758,377 + +2.1
4,758,444 - -0.2
4,758,490 - +0.2
4,758,490 - +2.6
4,758,520 - -1.4
4,758,561 + -1.7
4,758,569 - +0.1
4,758,608 + +0.6
4,758,672 + -0.2
4,758,704 - -0.9
4,758,891 - BWI76_RS22815 0.12 -0.8
4,758,959 - BWI76_RS22815 0.15 -0.7
4,759,116 - BWI76_RS22815 0.20 -0.5
4,759,214 + BWI76_RS22815 0.24 -1.8
4,759,234 - BWI76_RS22815 0.25 -0.6
4,759,482 - BWI76_RS22815 0.34 +3.1
4,759,595 + BWI76_RS22815 0.38 -0.9
4,759,670 - BWI76_RS22815 0.41 -1.3
4,759,685 - BWI76_RS22815 0.41 -1.3
4,759,708 - BWI76_RS22815 0.42 -1.9
4,759,713 + BWI76_RS22815 0.42 +0.6
4,760,066 - BWI76_RS22815 0.55 -0.8
4,760,092 - BWI76_RS22815 0.56 -0.6
4,760,103 + BWI76_RS22815 0.56 -0.1
4,760,126 + BWI76_RS22815 0.57 -0.9
4,760,134 - BWI76_RS22815 0.57 -0.2
4,760,149 + BWI76_RS22815 0.58 -0.0
4,760,168 - BWI76_RS22815 0.59 +0.7
4,760,330 - BWI76_RS22815 0.64 -2.5
4,760,412 - BWI76_RS22815 0.67 -1.6
4,760,606 + BWI76_RS22815 0.75 +0.9
4,760,646 + BWI76_RS22815 0.76 -0.9
4,761,020 - BWI76_RS22815 0.90 -2.0
4,761,096 - -1.4
4,761,103 + -0.3
4,761,328 - -0.0
4,761,435 - -0.0
4,761,571 + BWI76_RS22820 0.20 +1.9
4,761,781 - BWI76_RS22820 0.38 +2.2
4,761,943 + BWI76_RS22820 0.52 +0.7
4,761,978 + BWI76_RS22820 0.55 -0.3
4,761,978 + BWI76_RS22820 0.55 -0.2
4,761,986 - BWI76_RS22820 0.56 -3.1

Or see this region's nucleotide sequence