Experiment: KLEB29 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22735 and BWI76_RS22740 are separated by 14 nucleotides BWI76_RS22740 and BWI76_RS22745 overlap by 4 nucleotides BWI76_RS22745 and BWI76_RS22750 are separated by 11 nucleotides
BWI76_RS22735: BWI76_RS22735 - type I-E CRISPR-associated protein Cas7/Cse4/CasC, at 4,737,105 to 4,738,163
_RS22735
BWI76_RS22740: BWI76_RS22740 - type I-E CRISPR-associated protein Cse2/CasB, at 4,738,178 to 4,738,729
_RS22740
BWI76_RS22745: BWI76_RS22745 - type I-E CRISPR-associated protein Cse1/CasA, at 4,738,726 to 4,740,285
_RS22745
BWI76_RS22750: BWI76_RS22750 - CRISPR-associated helicase/endonuclease Cas3, at 4,740,297 to 4,742,957
_RS22750
Position (kb)
4738
4739
4740
4741 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 4737.806 kb on - strand, within BWI76_RS22735 at 4737.956 kb on + strand, within BWI76_RS22735 at 4738.074 kb on - strand at 4738.243 kb on - strand, within BWI76_RS22740 at 4738.264 kb on + strand, within BWI76_RS22740 at 4738.359 kb on + strand, within BWI76_RS22740 at 4738.359 kb on + strand, within BWI76_RS22740 at 4738.367 kb on - strand, within BWI76_RS22740 at 4738.367 kb on - strand, within BWI76_RS22740 at 4738.431 kb on - strand, within BWI76_RS22740 at 4738.494 kb on + strand, within BWI76_RS22740 at 4738.549 kb on + strand, within BWI76_RS22740 at 4738.701 kb on + strand at 4738.704 kb on - strand at 4738.846 kb on - strand at 4738.853 kb on + strand at 4739.070 kb on + strand, within BWI76_RS22745 at 4739.070 kb on + strand, within BWI76_RS22745 at 4739.070 kb on + strand, within BWI76_RS22745 at 4739.070 kb on + strand at 4739.070 kb on + strand, within BWI76_RS22745 at 4739.070 kb on + strand, within BWI76_RS22745 at 4739.070 kb on + strand, within BWI76_RS22745 at 4739.078 kb on - strand, within BWI76_RS22745 at 4739.078 kb on - strand, within BWI76_RS22745 at 4739.078 kb on - strand at 4739.078 kb on - strand, within BWI76_RS22745 at 4739.078 kb on - strand, within BWI76_RS22745 at 4739.159 kb on - strand, within BWI76_RS22745 at 4739.431 kb on - strand, within BWI76_RS22745 at 4739.490 kb on - strand, within BWI76_RS22745 at 4739.540 kb on - strand, within BWI76_RS22745 at 4739.550 kb on - strand, within BWI76_RS22745 at 4739.585 kb on + strand, within BWI76_RS22745 at 4739.593 kb on - strand, within BWI76_RS22745 at 4739.593 kb on - strand, within BWI76_RS22745 at 4739.598 kb on - strand, within BWI76_RS22745 at 4739.641 kb on - strand, within BWI76_RS22745 at 4739.663 kb on + strand, within BWI76_RS22745 at 4739.671 kb on - strand, within BWI76_RS22745 at 4739.763 kb on - strand, within BWI76_RS22745 at 4739.795 kb on - strand, within BWI76_RS22745 at 4739.806 kb on - strand, within BWI76_RS22745 at 4739.913 kb on + strand, within BWI76_RS22745 at 4740.056 kb on - strand, within BWI76_RS22745 at 4740.056 kb on - strand, within BWI76_RS22745 at 4740.056 kb on - strand, within BWI76_RS22745 at 4740.115 kb on - strand, within BWI76_RS22745 at 4740.127 kb on - strand, within BWI76_RS22745 at 4740.151 kb on + strand at 4740.159 kb on - strand at 4740.416 kb on - strand at 4740.476 kb on - strand at 4740.476 kb on - strand at 4740.476 kb on - strand at 4740.509 kb on + strand at 4740.509 kb on + strand at 4740.523 kb on + strand at 4740.617 kb on + strand, within BWI76_RS22750 at 4740.814 kb on - strand, within BWI76_RS22750 at 4740.858 kb on + strand, within BWI76_RS22750 at 4740.918 kb on + strand, within BWI76_RS22750 at 4741.093 kb on - strand, within BWI76_RS22750 at 4741.096 kb on + strand, within BWI76_RS22750 at 4741.103 kb on - strand at 4741.220 kb on + strand, within BWI76_RS22750 at 4741.220 kb on + strand, within BWI76_RS22750 at 4741.220 kb on + strand, within BWI76_RS22750 at 4741.226 kb on - strand, within BWI76_RS22750 at 4741.228 kb on - strand, within BWI76_RS22750 at 4741.228 kb on - strand, within BWI76_RS22750 at 4741.233 kb on + strand, within BWI76_RS22750 at 4741.241 kb on - strand, within BWI76_RS22750
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB29 MOI 1 remove 4,737,806 - BWI76_RS22735 0.66 -0.4 4,737,956 + BWI76_RS22735 0.80 +0.3 4,738,074 - -0.7 4,738,243 - BWI76_RS22740 0.12 +0.2 4,738,264 + BWI76_RS22740 0.16 -0.1 4,738,359 + BWI76_RS22740 0.33 -0.7 4,738,359 + BWI76_RS22740 0.33 -1.2 4,738,367 - BWI76_RS22740 0.34 -0.6 4,738,367 - BWI76_RS22740 0.34 +0.4 4,738,431 - BWI76_RS22740 0.46 -0.6 4,738,494 + BWI76_RS22740 0.57 -0.1 4,738,549 + BWI76_RS22740 0.67 +1.3 4,738,701 + +0.4 4,738,704 - -0.5 4,738,846 - +0.3 4,738,853 + +0.2 4,739,070 + BWI76_RS22745 0.22 -0.4 4,739,070 + BWI76_RS22745 0.22 +0.8 4,739,070 + BWI76_RS22745 0.22 -0.4 4,739,070 + +0.1 4,739,070 + BWI76_RS22745 0.22 +0.6 4,739,070 + BWI76_RS22745 0.22 +0.5 4,739,070 + BWI76_RS22745 0.22 +0.1 4,739,078 - BWI76_RS22745 0.23 +0.3 4,739,078 - BWI76_RS22745 0.23 +0.4 4,739,078 - +1.1 4,739,078 - BWI76_RS22745 0.23 -1.3 4,739,078 - BWI76_RS22745 0.23 +0.2 4,739,159 - BWI76_RS22745 0.28 -3.0 4,739,431 - BWI76_RS22745 0.45 +0.5 4,739,490 - BWI76_RS22745 0.49 +0.8 4,739,540 - BWI76_RS22745 0.52 -0.1 4,739,550 - BWI76_RS22745 0.53 -1.5 4,739,585 + BWI76_RS22745 0.55 +1.7 4,739,593 - BWI76_RS22745 0.56 -0.4 4,739,593 - BWI76_RS22745 0.56 +0.2 4,739,598 - BWI76_RS22745 0.56 -0.9 4,739,641 - BWI76_RS22745 0.59 +2.3 4,739,663 + BWI76_RS22745 0.60 +1.0 4,739,671 - BWI76_RS22745 0.61 +0.7 4,739,763 - BWI76_RS22745 0.66 -0.1 4,739,795 - BWI76_RS22745 0.69 -0.2 4,739,806 - BWI76_RS22745 0.69 +1.1 4,739,913 + BWI76_RS22745 0.76 -2.5 4,740,056 - BWI76_RS22745 0.85 +1.0 4,740,056 - BWI76_RS22745 0.85 +1.4 4,740,056 - BWI76_RS22745 0.85 -0.7 4,740,115 - BWI76_RS22745 0.89 -0.2 4,740,127 - BWI76_RS22745 0.90 +4.1 4,740,151 + +0.3 4,740,159 - -1.9 4,740,416 - -0.1 4,740,476 - +0.2 4,740,476 - +0.3 4,740,476 - -0.0 4,740,509 + +1.9 4,740,509 + +1.1 4,740,523 + +0.9 4,740,617 + BWI76_RS22750 0.12 -2.9 4,740,814 - BWI76_RS22750 0.19 -0.3 4,740,858 + BWI76_RS22750 0.21 -0.7 4,740,918 + BWI76_RS22750 0.23 -1.1 4,741,093 - BWI76_RS22750 0.30 -1.1 4,741,096 + BWI76_RS22750 0.30 -2.1 4,741,103 - -1.3 4,741,220 + BWI76_RS22750 0.35 +0.0 4,741,220 + BWI76_RS22750 0.35 +1.2 4,741,220 + BWI76_RS22750 0.35 -1.3 4,741,226 - BWI76_RS22750 0.35 +1.5 4,741,228 - BWI76_RS22750 0.35 -1.3 4,741,228 - BWI76_RS22750 0.35 +1.1 4,741,233 + BWI76_RS22750 0.35 -2.3 4,741,241 - BWI76_RS22750 0.35 -0.4
Or see this region's nucleotide sequence