Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS22300

Experiment: KLEB29 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS22295 and BWI76_RS22300 are separated by 212 nucleotidesBWI76_RS22300 and BWI76_RS22305 overlap by 8 nucleotidesBWI76_RS22305 and BWI76_RS22310 overlap by 4 nucleotides BWI76_RS22295: BWI76_RS22295 - 6-phospho-beta-glucosidase, at 4,648,310 to 4,649,734 _RS22295 BWI76_RS22300: BWI76_RS22300 - hydrogenase 3 maturation endopeptidase HyCI, at 4,649,947 to 4,650,432 _RS22300 BWI76_RS22305: BWI76_RS22305 - formate hydrogenlyase maturation protein HycH, at 4,650,425 to 4,650,835 _RS22305 BWI76_RS22310: BWI76_RS22310 - hydrogenase 3 small subunit, at 4,650,832 to 4,651,599 _RS22310 Position (kb) 4649 4650 4651Strain fitness (log2 ratio) -1 0 1at 4649.018 kb on - strand, within BWI76_RS22295at 4649.040 kb on - strand, within BWI76_RS22295at 4649.119 kb on - strand, within BWI76_RS22295at 4649.173 kb on - strand, within BWI76_RS22295at 4649.251 kb on - strand, within BWI76_RS22295at 4649.368 kb on + strand, within BWI76_RS22295at 4649.552 kb on + strand, within BWI76_RS22295at 4649.559 kb on - strand, within BWI76_RS22295at 4649.614 kb on + strandat 4649.774 kb on - strandat 4649.995 kb on + strandat 4650.366 kb on - strand, within BWI76_RS22300at 4650.494 kb on - strand, within BWI76_RS22305at 4650.990 kb on + strand, within BWI76_RS22310at 4651.316 kb on + strand, within BWI76_RS22310at 4651.334 kb on - strand, within BWI76_RS22310at 4651.414 kb on - strand, within BWI76_RS22310at 4651.414 kb on - strand, within BWI76_RS22310

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB29 MOI 1
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4,649,018 - BWI76_RS22295 0.50 +0.9
4,649,040 - BWI76_RS22295 0.51 -0.2
4,649,119 - BWI76_RS22295 0.57 +0.2
4,649,173 - BWI76_RS22295 0.61 +0.6
4,649,251 - BWI76_RS22295 0.66 +0.0
4,649,368 + BWI76_RS22295 0.74 +0.1
4,649,552 + BWI76_RS22295 0.87 -1.5
4,649,559 - BWI76_RS22295 0.88 +0.7
4,649,614 + -1.3
4,649,774 - +0.2
4,649,995 + -0.6
4,650,366 - BWI76_RS22300 0.86 -1.4
4,650,494 - BWI76_RS22305 0.17 +0.7
4,650,990 + BWI76_RS22310 0.21 +0.7
4,651,316 + BWI76_RS22310 0.63 +0.4
4,651,334 - BWI76_RS22310 0.65 -0.3
4,651,414 - BWI76_RS22310 0.76 +0.8
4,651,414 - BWI76_RS22310 0.76 -1.6

Or see this region's nucleotide sequence