Experiment: KLEB29 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18860 and BWI76_RS18865 overlap by 1 nucleotides BWI76_RS18865 and BWI76_RS18870 overlap by 4 nucleotides BWI76_RS18870 and BWI76_RS18875 overlap by 4 nucleotides BWI76_RS18875 and BWI76_RS18880 are separated by 23 nucleotides
BWI76_RS18860: BWI76_RS18860 - homocitrate synthase, at 3,912,103 to 3,913,245
_RS18860
BWI76_RS18865: BWI76_RS18865 - nitrogenase-stabilizing/protective protein NifW, at 3,913,245 to 3,913,505
_RS18865
BWI76_RS18870: BWI76_RS18870 - protein NifZ, at 3,913,502 to 3,913,948
_RS18870
BWI76_RS18875: BWI76_RS18875 - nitrogen fixation protein NifM, at 3,913,945 to 3,914,745
_RS18875
BWI76_RS18880: BWI76_RS18880 - flavodoxin, at 3,914,769 to 3,915,299
_RS18880
Position (kb)
3913
3914 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3912.636 kb on + strand, within BWI76_RS18860 at 3912.750 kb on + strand, within BWI76_RS18860 at 3912.954 kb on - strand, within BWI76_RS18860 at 3913.047 kb on + strand, within BWI76_RS18860 at 3913.104 kb on + strand, within BWI76_RS18860 at 3913.119 kb on + strand, within BWI76_RS18860 at 3913.380 kb on - strand, within BWI76_RS18865 at 3913.774 kb on - strand, within BWI76_RS18870 at 3913.910 kb on + strand at 3914.056 kb on + strand, within BWI76_RS18875 at 3914.312 kb on - strand, within BWI76_RS18875 at 3914.368 kb on + strand, within BWI76_RS18875 at 3914.464 kb on - strand, within BWI76_RS18875 at 3914.498 kb on - strand, within BWI76_RS18875 at 3914.598 kb on - strand, within BWI76_RS18875 at 3914.792 kb on + strand at 3914.813 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB29 MOI 1 remove 3,912,636 + BWI76_RS18860 0.47 -1.8 3,912,750 + BWI76_RS18860 0.57 +2.0 3,912,954 - BWI76_RS18860 0.74 +0.8 3,913,047 + BWI76_RS18860 0.83 +0.6 3,913,104 + BWI76_RS18860 0.88 +0.8 3,913,119 + BWI76_RS18860 0.89 +0.1 3,913,380 - BWI76_RS18865 0.52 -0.8 3,913,774 - BWI76_RS18870 0.61 +1.1 3,913,910 + -3.0 3,914,056 + BWI76_RS18875 0.14 +0.3 3,914,312 - BWI76_RS18875 0.46 +0.1 3,914,368 + BWI76_RS18875 0.53 +2.6 3,914,464 - BWI76_RS18875 0.65 +0.3 3,914,498 - BWI76_RS18875 0.69 -0.0 3,914,598 - BWI76_RS18875 0.82 +0.8 3,914,792 + +0.1 3,914,813 - +0.4
Or see this region's nucleotide sequence