Experiment: KLEB29 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17770 and BWI76_RS17775 are separated by 16 nucleotides BWI76_RS17775 and BWI76_RS17780 are separated by 126 nucleotides BWI76_RS17780 and BWI76_RS17785 are separated by 23 nucleotides BWI76_RS17785 and BWI76_RS17790 are separated by 3 nucleotides
BWI76_RS17770: BWI76_RS17770 - isomerase/hydrolase, at 3,694,972 to 3,695,631
_RS17770
BWI76_RS17775: BWI76_RS17775 - hypothetical protein, at 3,695,648 to 3,695,929
_RS17775
BWI76_RS17780: BWI76_RS17780 - septum formation inhibitor, at 3,696,056 to 3,696,754
_RS17780
BWI76_RS17785: BWI76_RS17785 - septum site-determining protein MinD, at 3,696,778 to 3,697,590
_RS17785
BWI76_RS17790: BWI76_RS17790 - cell division topological specificity factor, at 3,697,594 to 3,697,863
_RS17790
Position (kb)
3696
3697 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3695.186 kb on - strand, within BWI76_RS17770 at 3695.420 kb on + strand, within BWI76_RS17770 at 3695.614 kb on + strand at 3695.614 kb on + strand at 3695.614 kb on + strand at 3695.646 kb on - strand at 3695.668 kb on + strand at 3695.721 kb on + strand, within BWI76_RS17775 at 3695.821 kb on + strand, within BWI76_RS17775 at 3695.966 kb on + strand at 3695.968 kb on + strand at 3695.968 kb on + strand at 3695.976 kb on - strand at 3696.017 kb on - strand at 3696.043 kb on + strand at 3696.089 kb on + strand at 3696.097 kb on - strand at 3696.200 kb on - strand, within BWI76_RS17780 at 3696.379 kb on + strand, within BWI76_RS17780 at 3696.456 kb on + strand, within BWI76_RS17780 at 3696.474 kb on - strand, within BWI76_RS17780 at 3696.544 kb on + strand, within BWI76_RS17780 at 3696.720 kb on - strand at 3696.851 kb on - strand at 3696.914 kb on - strand, within BWI76_RS17785 at 3697.023 kb on + strand, within BWI76_RS17785 at 3697.078 kb on + strand, within BWI76_RS17785 at 3697.086 kb on - strand, within BWI76_RS17785 at 3697.210 kb on - strand, within BWI76_RS17785 at 3697.436 kb on + strand, within BWI76_RS17785 at 3697.436 kb on + strand, within BWI76_RS17785 at 3697.436 kb on + strand, within BWI76_RS17785 at 3697.444 kb on - strand, within BWI76_RS17785
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB29 MOI 1 remove 3,695,186 - BWI76_RS17770 0.32 -0.2 3,695,420 + BWI76_RS17770 0.68 +1.7 3,695,614 + -0.1 3,695,614 + +1.0 3,695,614 + +0.4 3,695,646 - +0.6 3,695,668 + -1.0 3,695,721 + BWI76_RS17775 0.26 +1.5 3,695,821 + BWI76_RS17775 0.61 +1.2 3,695,966 + +0.0 3,695,968 + -0.1 3,695,968 + -1.6 3,695,976 - +1.3 3,696,017 - +3.2 3,696,043 + +0.2 3,696,089 + -1.0 3,696,097 - -2.3 3,696,200 - BWI76_RS17780 0.21 -2.1 3,696,379 + BWI76_RS17780 0.46 -1.6 3,696,456 + BWI76_RS17780 0.57 +0.1 3,696,474 - BWI76_RS17780 0.60 -3.7 3,696,544 + BWI76_RS17780 0.70 -0.1 3,696,720 - -3.7 3,696,851 - -2.0 3,696,914 - BWI76_RS17785 0.17 -2.0 3,697,023 + BWI76_RS17785 0.30 -1.2 3,697,078 + BWI76_RS17785 0.37 -2.3 3,697,086 - BWI76_RS17785 0.38 +0.2 3,697,210 - BWI76_RS17785 0.53 -0.6 3,697,436 + BWI76_RS17785 0.81 -0.2 3,697,436 + BWI76_RS17785 0.81 -1.5 3,697,436 + BWI76_RS17785 0.81 +1.6 3,697,444 - BWI76_RS17785 0.82 +0.6
Or see this region's nucleotide sequence