Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS17210

Experiment: KLEB29 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS17205 and BWI76_RS17210 are separated by 34 nucleotidesBWI76_RS17210 and BWI76_RS17215 are separated by 67 nucleotidesBWI76_RS17215 and BWI76_RS17220 are separated by 45 nucleotides BWI76_RS17205: BWI76_RS17205 - cardiolipin synthase A, at 3,563,938 to 3,565,398 _RS17205 BWI76_RS17210: BWI76_RS17210 - dsDNA-mimic protein, at 3,565,433 to 3,565,762 _RS17210 BWI76_RS17215: BWI76_RS17215 - transporter, at 3,565,830 to 3,566,667 _RS17215 BWI76_RS17220: BWI76_RS17220 - oligopeptide ABC transporter ATP-binding protein OppF, at 3,566,713 to 3,567,717 _RS17220 Position (kb) 3565 3566Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 3564.598 kb on - strand, within BWI76_RS17205at 3564.913 kb on - strand, within BWI76_RS17205at 3565.201 kb on - strand, within BWI76_RS17205at 3565.325 kb on + strandat 3565.400 kb on - strandat 3565.600 kb on - strand, within BWI76_RS17210at 3565.602 kb on + strand, within BWI76_RS17210at 3565.607 kb on - strand, within BWI76_RS17210at 3565.629 kb on + strand, within BWI76_RS17210at 3565.658 kb on + strand, within BWI76_RS17210at 3565.702 kb on - strand, within BWI76_RS17210at 3565.735 kb on - strandat 3565.740 kb on + strandat 3565.740 kb on + strandat 3565.740 kb on + strandat 3565.740 kb on + strandat 3565.740 kb on + strandat 3565.740 kb on + strandat 3565.740 kb on + strandat 3565.740 kb on + strandat 3565.740 kb on + strandat 3565.740 kb on + strandat 3565.740 kb on + strandat 3565.740 kb on + strandat 3565.740 kb on + strandat 3565.748 kb on - strandat 3565.748 kb on - strandat 3565.748 kb on - strandat 3565.748 kb on - strandat 3565.748 kb on - strandat 3565.748 kb on - strandat 3565.798 kb on - strandat 3565.841 kb on + strandat 3565.908 kb on + strandat 3565.916 kb on + strand, within BWI76_RS17215at 3565.916 kb on + strand, within BWI76_RS17215at 3565.936 kb on + strand, within BWI76_RS17215at 3566.006 kb on - strand, within BWI76_RS17215at 3566.034 kb on - strand, within BWI76_RS17215at 3566.197 kb on + strand, within BWI76_RS17215at 3566.198 kb on - strand, within BWI76_RS17215at 3566.207 kb on + strand, within BWI76_RS17215at 3566.210 kb on - strand, within BWI76_RS17215at 3566.215 kb on - strand, within BWI76_RS17215at 3566.220 kb on - strand, within BWI76_RS17215at 3566.244 kb on + strand, within BWI76_RS17215at 3566.259 kb on + strand, within BWI76_RS17215at 3566.321 kb on - strand, within BWI76_RS17215at 3566.323 kb on + strand, within BWI76_RS17215at 3566.325 kb on + strand, within BWI76_RS17215at 3566.326 kb on - strand, within BWI76_RS17215at 3566.345 kb on + strand, within BWI76_RS17215at 3566.433 kb on + strand, within BWI76_RS17215at 3566.441 kb on - strand, within BWI76_RS17215at 3566.469 kb on + strand, within BWI76_RS17215at 3566.477 kb on - strand, within BWI76_RS17215at 3566.560 kb on - strand, within BWI76_RS17215at 3566.560 kb on - strand, within BWI76_RS17215at 3566.560 kb on - strand, within BWI76_RS17215at 3566.562 kb on + strand, within BWI76_RS17215at 3566.700 kb on - strandat 3566.719 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB29 MOI 1
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3,564,598 - BWI76_RS17205 0.45 +0.6
3,564,913 - BWI76_RS17205 0.67 +3.0
3,565,201 - BWI76_RS17205 0.86 +0.8
3,565,325 + +2.4
3,565,400 - +0.6
3,565,600 - BWI76_RS17210 0.51 -1.4
3,565,602 + BWI76_RS17210 0.51 -1.0
3,565,607 - BWI76_RS17210 0.53 -1.3
3,565,629 + BWI76_RS17210 0.59 -0.6
3,565,658 + BWI76_RS17210 0.68 -2.3
3,565,702 - BWI76_RS17210 0.82 -0.2
3,565,735 - +4.2
3,565,740 + -1.9
3,565,740 + +0.8
3,565,740 + +0.2
3,565,740 + +0.1
3,565,740 + +0.1
3,565,740 + +1.0
3,565,740 + +0.4
3,565,740 + -1.6
3,565,740 + -0.4
3,565,740 + -0.4
3,565,740 + -0.9
3,565,740 + +1.0
3,565,740 + +0.1
3,565,748 - -0.3
3,565,748 - -0.3
3,565,748 - -0.1
3,565,748 - -0.1
3,565,748 - +0.0
3,565,748 - -0.6
3,565,798 - -1.0
3,565,841 + -0.3
3,565,908 + +2.2
3,565,916 + BWI76_RS17215 0.10 +0.9
3,565,916 + BWI76_RS17215 0.10 -1.3
3,565,936 + BWI76_RS17215 0.13 +1.0
3,566,006 - BWI76_RS17215 0.21 +0.6
3,566,034 - BWI76_RS17215 0.24 +1.2
3,566,197 + BWI76_RS17215 0.44 -1.0
3,566,198 - BWI76_RS17215 0.44 +0.2
3,566,207 + BWI76_RS17215 0.45 +0.2
3,566,210 - BWI76_RS17215 0.45 +2.2
3,566,215 - BWI76_RS17215 0.46 +2.0
3,566,220 - BWI76_RS17215 0.47 +0.6
3,566,244 + BWI76_RS17215 0.49 +0.5
3,566,259 + BWI76_RS17215 0.51 -0.3
3,566,321 - BWI76_RS17215 0.59 -2.7
3,566,323 + BWI76_RS17215 0.59 +0.5
3,566,325 + BWI76_RS17215 0.59 +1.0
3,566,326 - BWI76_RS17215 0.59 +2.7
3,566,345 + BWI76_RS17215 0.61 +2.5
3,566,433 + BWI76_RS17215 0.72 -0.5
3,566,441 - BWI76_RS17215 0.73 +0.1
3,566,469 + BWI76_RS17215 0.76 -0.4
3,566,477 - BWI76_RS17215 0.77 +1.0
3,566,560 - BWI76_RS17215 0.87 -1.7
3,566,560 - BWI76_RS17215 0.87 +0.0
3,566,560 - BWI76_RS17215 0.87 +0.4
3,566,562 + BWI76_RS17215 0.87 +0.2
3,566,700 - -0.5
3,566,719 - +1.0

Or see this region's nucleotide sequence