Experiment: KLEB29 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16770 and BWI76_RS16775 are separated by 497 nucleotides BWI76_RS16775 and BWI76_RS16780 are separated by 237 nucleotides
BWI76_RS16770: BWI76_RS16770 - extracellular solute-binding protein, at 3,474,697 to 3,475,785
_RS16770
BWI76_RS16775: BWI76_RS16775 - malate/lactate/ureidoglycolate dehydrogenase, at 3,476,283 to 3,477,365
_RS16775
BWI76_RS16780: BWI76_RS16780 - formate dehydrogenase subunit alpha, at 3,477,603 to 3,479,753
_RS16780
Position (kb)
3476
3477
3478 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3475.456 kb on + strand, within BWI76_RS16770 at 3475.516 kb on - strand, within BWI76_RS16770 at 3475.645 kb on + strand, within BWI76_RS16770 at 3475.653 kb on - strand, within BWI76_RS16770 at 3475.653 kb on - strand, within BWI76_RS16770 at 3475.700 kb on + strand at 3475.706 kb on + strand at 3475.706 kb on + strand at 3475.714 kb on - strand at 3475.714 kb on - strand at 3475.716 kb on - strand at 3475.839 kb on + strand at 3475.847 kb on - strand at 3476.063 kb on - strand at 3476.064 kb on - strand at 3476.073 kb on + strand at 3476.083 kb on + strand at 3476.083 kb on + strand at 3476.083 kb on + strand at 3476.089 kb on + strand at 3476.090 kb on - strand at 3476.091 kb on - strand at 3476.097 kb on - strand at 3476.097 kb on - strand at 3476.204 kb on - strand at 3476.209 kb on + strand at 3476.209 kb on + strand at 3476.272 kb on + strand at 3476.314 kb on - strand at 3476.428 kb on + strand, within BWI76_RS16775 at 3476.480 kb on + strand, within BWI76_RS16775 at 3477.146 kb on + strand, within BWI76_RS16775 at 3477.180 kb on - strand, within BWI76_RS16775 at 3477.202 kb on + strand, within BWI76_RS16775 at 3477.533 kb on + strand at 3477.556 kb on - strand at 3477.591 kb on + strand at 3477.620 kb on + strand at 3478.000 kb on - strand, within BWI76_RS16780 at 3478.275 kb on + strand, within BWI76_RS16780 at 3478.323 kb on + strand, within BWI76_RS16780 at 3478.336 kb on - strand, within BWI76_RS16780
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB29 MOI 1 remove 3,475,456 + BWI76_RS16770 0.70 -0.1 3,475,516 - BWI76_RS16770 0.75 +2.8 3,475,645 + BWI76_RS16770 0.87 -0.8 3,475,653 - BWI76_RS16770 0.88 -1.1 3,475,653 - BWI76_RS16770 0.88 +0.8 3,475,700 + -1.3 3,475,706 + -0.4 3,475,706 + +0.8 3,475,714 - +1.1 3,475,714 - +0.3 3,475,716 - -1.5 3,475,839 + +0.4 3,475,847 - -0.7 3,476,063 - -0.8 3,476,064 - +0.4 3,476,073 + -1.1 3,476,083 + -2.2 3,476,083 + +1.1 3,476,083 + -0.5 3,476,089 + +1.4 3,476,090 - +1.2 3,476,091 - -0.5 3,476,097 - +0.6 3,476,097 - +0.1 3,476,204 - +1.1 3,476,209 + -0.1 3,476,209 + -2.1 3,476,272 + -1.9 3,476,314 - -0.0 3,476,428 + BWI76_RS16775 0.13 -1.8 3,476,480 + BWI76_RS16775 0.18 +0.5 3,477,146 + BWI76_RS16775 0.80 -2.6 3,477,180 - BWI76_RS16775 0.83 -0.9 3,477,202 + BWI76_RS16775 0.85 -0.5 3,477,533 + -0.5 3,477,556 - -0.5 3,477,591 + -0.2 3,477,620 + +0.8 3,478,000 - BWI76_RS16780 0.18 +1.1 3,478,275 + BWI76_RS16780 0.31 +0.6 3,478,323 + BWI76_RS16780 0.33 -0.8 3,478,336 - BWI76_RS16780 0.34 -0.7
Or see this region's nucleotide sequence