Experiment: KLEB29 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16025 and BWI76_RS16030 are separated by 17 nucleotides BWI76_RS16030 and BWI76_RS16035 overlap by 8 nucleotides BWI76_RS16035 and BWI76_RS16040 overlap by 1 nucleotides BWI76_RS16040 and BWI76_RS16045 are separated by 129 nucleotides
BWI76_RS16025: BWI76_RS16025 - triphosphoribosyl-dephospho-CoA synthase, at 3,323,772 to 3,324,614
_RS16025
BWI76_RS16030: BWI76_RS16030 - malonate decarboxylase acyl carrier protein, at 3,324,632 to 3,324,931
_RS16030
BWI76_RS16035: BWI76_RS16035 - biotin-independent malonate decarboxylase subunit beta, at 3,324,924 to 3,325,757
_RS16035
BWI76_RS16040: BWI76_RS16040 - biotin-independent malonate decarboxylase subunit gamma, at 3,325,757 to 3,326,557
_RS16040
BWI76_RS16045: BWI76_RS16045 - transporter, at 3,326,687 to 3,327,646
_RS16045
Position (kb)
3324
3325
3326 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3324.104 kb on - strand, within BWI76_RS16025 at 3324.304 kb on - strand, within BWI76_RS16025 at 3324.729 kb on - strand, within BWI76_RS16030 at 3324.776 kb on - strand, within BWI76_RS16030 at 3324.906 kb on + strand at 3324.991 kb on - strand at 3325.238 kb on + strand, within BWI76_RS16035 at 3325.287 kb on - strand, within BWI76_RS16035 at 3325.339 kb on + strand, within BWI76_RS16035 at 3326.371 kb on + strand, within BWI76_RS16040 at 3326.379 kb on - strand, within BWI76_RS16040 at 3326.517 kb on + strand at 3326.525 kb on - strand at 3326.580 kb on - strand at 3326.582 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB29 MOI 1 remove 3,324,104 - BWI76_RS16025 0.39 -1.2 3,324,304 - BWI76_RS16025 0.63 -1.0 3,324,729 - BWI76_RS16030 0.32 +2.7 3,324,776 - BWI76_RS16030 0.48 -0.1 3,324,906 + -1.7 3,324,991 - -0.1 3,325,238 + BWI76_RS16035 0.38 -1.1 3,325,287 - BWI76_RS16035 0.44 -2.0 3,325,339 + BWI76_RS16035 0.50 -3.1 3,326,371 + BWI76_RS16040 0.77 -0.2 3,326,379 - BWI76_RS16040 0.78 +0.8 3,326,517 + -0.4 3,326,525 - +1.1 3,326,580 - +1.1 3,326,582 + -0.3
Or see this region's nucleotide sequence