Experiment: KLEB29 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16020 and BWI76_RS16025 overlap by 1 nucleotides BWI76_RS16025 and BWI76_RS16030 are separated by 17 nucleotides BWI76_RS16030 and BWI76_RS16035 overlap by 8 nucleotides
BWI76_RS16020: BWI76_RS16020 - malonate decarboxylase subunit alpha, at 3,322,117 to 3,323,772
_RS16020
BWI76_RS16025: BWI76_RS16025 - triphosphoribosyl-dephospho-CoA synthase, at 3,323,772 to 3,324,614
_RS16025
BWI76_RS16030: BWI76_RS16030 - malonate decarboxylase acyl carrier protein, at 3,324,632 to 3,324,931
_RS16030
BWI76_RS16035: BWI76_RS16035 - biotin-independent malonate decarboxylase subunit beta, at 3,324,924 to 3,325,757
_RS16035
Position (kb)
3323
3324
3325 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3322.910 kb on + strand, within BWI76_RS16020 at 3322.965 kb on - strand, within BWI76_RS16020 at 3323.076 kb on - strand, within BWI76_RS16020 at 3323.385 kb on - strand, within BWI76_RS16020 at 3323.525 kb on - strand, within BWI76_RS16020 at 3323.737 kb on + strand at 3324.104 kb on - strand, within BWI76_RS16025 at 3324.304 kb on - strand, within BWI76_RS16025 at 3324.729 kb on - strand, within BWI76_RS16030 at 3324.776 kb on - strand, within BWI76_RS16030 at 3324.906 kb on + strand at 3324.991 kb on - strand at 3325.238 kb on + strand, within BWI76_RS16035 at 3325.287 kb on - strand, within BWI76_RS16035 at 3325.339 kb on + strand, within BWI76_RS16035
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB29 MOI 1 remove 3,322,910 + BWI76_RS16020 0.48 +0.0 3,322,965 - BWI76_RS16020 0.51 +4.2 3,323,076 - BWI76_RS16020 0.58 +1.0 3,323,385 - BWI76_RS16020 0.77 +0.4 3,323,525 - BWI76_RS16020 0.85 -0.9 3,323,737 + +1.2 3,324,104 - BWI76_RS16025 0.39 -1.2 3,324,304 - BWI76_RS16025 0.63 -1.0 3,324,729 - BWI76_RS16030 0.32 +2.7 3,324,776 - BWI76_RS16030 0.48 -0.1 3,324,906 + -1.7 3,324,991 - -0.1 3,325,238 + BWI76_RS16035 0.38 -1.1 3,325,287 - BWI76_RS16035 0.44 -2.0 3,325,339 + BWI76_RS16035 0.50 -3.1
Or see this region's nucleotide sequence