Experiment: KLEB29 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14780 and BWI76_RS14785 are separated by 435 nucleotides BWI76_RS14785 and BWI76_RS14790 are separated by 36 nucleotides BWI76_RS14790 and BWI76_RS14795 are separated by 146 nucleotides BWI76_RS14795 and BWI76_RS14800 are separated by 45 nucleotides
BWI76_RS14780: BWI76_RS14780 - phosphotriesterase, at 3,099,517 to 3,100,488
_RS14780
BWI76_RS14785: BWI76_RS14785 - hypothetical protein, at 3,100,924 to 3,101,172
_RS14785
BWI76_RS14790: BWI76_RS14790 - 2-dehydropantoate 2-reductase, at 3,101,209 to 3,102,102
_RS14790
BWI76_RS14795: BWI76_RS14795 - hypothetical protein, at 3,102,249 to 3,102,473
_RS14795
BWI76_RS14800: BWI76_RS14800 - non-heme chloroperoxidase, at 3,102,519 to 3,103,355
_RS14800
Position (kb) 3101
3102
3103 Strain fitness (log2 ratio) -1
0
1 at 3100.627 kb on + strand at 3101.321 kb on - strand at 3101.463 kb on + strand, within BWI76_RS14790 at 3101.607 kb on + strand, within BWI76_RS14790 at 3102.083 kb on - strand at 3102.467 kb on + strand at 3102.467 kb on + strand at 3102.832 kb on - strand, within BWI76_RS14800 at 3102.995 kb on - strand, within BWI76_RS14800 at 3103.092 kb on - strand, within BWI76_RS14800
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB29 MOI 1 remove 3,100,627 + -0.1 3,101,321 - +1.3 3,101,463 + BWI76_RS14790 0.28 +0.3 3,101,607 + BWI76_RS14790 0.45 -0.5 3,102,083 - -0.9 3,102,467 + +0.2 3,102,467 + -0.1 3,102,832 - BWI76_RS14800 0.37 -0.5 3,102,995 - BWI76_RS14800 0.57 -0.0 3,103,092 - BWI76_RS14800 0.68 +0.9
Or see this region's nucleotide sequence