Experiment: KLEB29 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11330 and BWI76_RS11335 are separated by 156 nucleotides BWI76_RS11335 and BWI76_RS11340 are separated by 5 nucleotides BWI76_RS11340 and BWI76_RS11345 are separated by 100 nucleotides
BWI76_RS11330: BWI76_RS11330 - isocitrate dehydrogenase (NADP(+)), at 2,387,817 to 2,389,067
_RS11330
BWI76_RS11335: BWI76_RS11335 - tautomerase, at 2,389,224 to 2,389,610
_RS11335
BWI76_RS11340: BWI76_RS11340 - carboxymuconolactone decarboxylase, at 2,389,616 to 2,389,918
_RS11340
BWI76_RS11345: BWI76_RS11345 - LysR family transcriptional regulator, at 2,390,019 to 2,390,903
_RS11345
Position (kb)
2389
2390 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2389.183 kb on - strand at 2389.230 kb on - strand at 2389.232 kb on + strand at 2389.346 kb on + strand, within BWI76_RS11335 at 2389.581 kb on - strand at 2389.613 kb on + strand at 2389.748 kb on - strand at 2390.229 kb on + strand, within BWI76_RS11345 at 2390.595 kb on + strand, within BWI76_RS11345 at 2390.605 kb on + strand, within BWI76_RS11345
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB29 MOI 1 remove 2,389,183 - +0.6 2,389,230 - -0.3 2,389,232 + +2.1 2,389,346 + BWI76_RS11335 0.32 -3.3 2,389,581 - -0.2 2,389,613 + -0.7 2,389,748 - -0.1 2,390,229 + BWI76_RS11345 0.24 +0.8 2,390,595 + BWI76_RS11345 0.65 -1.0 2,390,605 + BWI76_RS11345 0.66 -1.8
Or see this region's nucleotide sequence