Experiment: KLEB29 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11175 and BWI76_RS11180 are separated by 225 nucleotides BWI76_RS11180 and BWI76_RS11185 are separated by 56 nucleotides BWI76_RS11185 and BWI76_RS11190 are separated by 353 nucleotides
BWI76_RS11175: BWI76_RS11175 - NADH dehydrogenase, at 2,354,672 to 2,355,976
_RS11175
BWI76_RS11180: BWI76_RS11180 - hypothetical protein, at 2,356,202 to 2,356,741
_RS11180
BWI76_RS11185: BWI76_RS11185 - ligand-gated channel protein, at 2,356,798 to 2,358,975
_RS11185
BWI76_RS11190: BWI76_RS11190 - putative TetR/AcrR family transcriptional regulatory protein, at 2,359,329 to 2,359,964
_RS11190
Position (kb)
2356
2357
2358
2359 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2356.272 kb on + strand, within BWI76_RS11180 at 2356.313 kb on + strand, within BWI76_RS11180 at 2356.561 kb on + strand, within BWI76_RS11180 at 2356.707 kb on + strand at 2356.867 kb on + strand at 2356.880 kb on - strand at 2357.384 kb on - strand, within BWI76_RS11185 at 2357.777 kb on - strand, within BWI76_RS11185 at 2357.913 kb on + strand, within BWI76_RS11185 at 2358.080 kb on - strand, within BWI76_RS11185 at 2358.208 kb on + strand, within BWI76_RS11185 at 2358.678 kb on - strand, within BWI76_RS11185 at 2359.082 kb on + strand at 2359.090 kb on - strand at 2359.095 kb on - strand at 2359.256 kb on - strand at 2359.399 kb on + strand, within BWI76_RS11190 at 2359.895 kb on - strand, within BWI76_RS11190
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB29 MOI 1 remove 2,356,272 + BWI76_RS11180 0.13 +0.9 2,356,313 + BWI76_RS11180 0.21 +0.1 2,356,561 + BWI76_RS11180 0.66 +1.5 2,356,707 + +0.4 2,356,867 + +2.0 2,356,880 - -1.9 2,357,384 - BWI76_RS11185 0.27 -1.3 2,357,777 - BWI76_RS11185 0.45 -0.5 2,357,913 + BWI76_RS11185 0.51 -0.1 2,358,080 - BWI76_RS11185 0.59 -1.0 2,358,208 + BWI76_RS11185 0.65 -0.6 2,358,678 - BWI76_RS11185 0.86 -1.8 2,359,082 + -0.4 2,359,090 - -1.5 2,359,095 - +0.7 2,359,256 - +2.5 2,359,399 + BWI76_RS11190 0.11 +1.9 2,359,895 - BWI76_RS11190 0.89 +2.1
Or see this region's nucleotide sequence