Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS11185

Experiment: KLEB29 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS11175 and BWI76_RS11180 are separated by 225 nucleotidesBWI76_RS11180 and BWI76_RS11185 are separated by 56 nucleotidesBWI76_RS11185 and BWI76_RS11190 are separated by 353 nucleotides BWI76_RS11175: BWI76_RS11175 - NADH dehydrogenase, at 2,354,672 to 2,355,976 _RS11175 BWI76_RS11180: BWI76_RS11180 - hypothetical protein, at 2,356,202 to 2,356,741 _RS11180 BWI76_RS11185: BWI76_RS11185 - ligand-gated channel protein, at 2,356,798 to 2,358,975 _RS11185 BWI76_RS11190: BWI76_RS11190 - putative TetR/AcrR family transcriptional regulatory protein, at 2,359,329 to 2,359,964 _RS11190 Position (kb) 2356 2357 2358 2359Strain fitness (log2 ratio) -2 -1 0 1 2at 2356.272 kb on + strand, within BWI76_RS11180at 2356.313 kb on + strand, within BWI76_RS11180at 2356.561 kb on + strand, within BWI76_RS11180at 2356.707 kb on + strandat 2356.867 kb on + strandat 2356.880 kb on - strandat 2357.384 kb on - strand, within BWI76_RS11185at 2357.777 kb on - strand, within BWI76_RS11185at 2357.913 kb on + strand, within BWI76_RS11185at 2358.080 kb on - strand, within BWI76_RS11185at 2358.208 kb on + strand, within BWI76_RS11185at 2358.678 kb on - strand, within BWI76_RS11185at 2359.082 kb on + strandat 2359.090 kb on - strandat 2359.095 kb on - strandat 2359.256 kb on - strandat 2359.399 kb on + strand, within BWI76_RS11190at 2359.895 kb on - strand, within BWI76_RS11190

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB29 MOI 1
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2,356,272 + BWI76_RS11180 0.13 +0.9
2,356,313 + BWI76_RS11180 0.21 +0.1
2,356,561 + BWI76_RS11180 0.66 +1.5
2,356,707 + +0.4
2,356,867 + +2.0
2,356,880 - -1.9
2,357,384 - BWI76_RS11185 0.27 -1.3
2,357,777 - BWI76_RS11185 0.45 -0.5
2,357,913 + BWI76_RS11185 0.51 -0.1
2,358,080 - BWI76_RS11185 0.59 -1.0
2,358,208 + BWI76_RS11185 0.65 -0.6
2,358,678 - BWI76_RS11185 0.86 -1.8
2,359,082 + -0.4
2,359,090 - -1.5
2,359,095 - +0.7
2,359,256 - +2.5
2,359,399 + BWI76_RS11190 0.11 +1.9
2,359,895 - BWI76_RS11190 0.89 +2.1

Or see this region's nucleotide sequence