Experiment: KLEB29 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11155 and BWI76_RS11160 overlap by 20 nucleotides BWI76_RS11160 and BWI76_RS11165 are separated by 19 nucleotides BWI76_RS11165 and BWI76_RS11170 are separated by 36 nucleotides BWI76_RS11170 and BWI76_RS11175 are separated by 233 nucleotides
BWI76_RS11155: BWI76_RS11155 - penicillin-binding protein activator LpoB, at 2,351,383 to 2,352,018
_RS11155
BWI76_RS11160: BWI76_RS11160 - thiamine kinase, at 2,351,999 to 2,352,817
_RS11160
BWI76_RS11165: BWI76_RS11165 - beta-N-acetylhexosaminidase, at 2,352,837 to 2,353,859
_RS11165
BWI76_RS11170: BWI76_RS11170 - hypothetical protein, at 2,353,896 to 2,354,438
_RS11170
BWI76_RS11175: BWI76_RS11175 - NADH dehydrogenase, at 2,354,672 to 2,355,976
_RS11175
Position (kb)
2352
2353
2354 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 2351.881 kb on - strand, within BWI76_RS11155 at 2352.007 kb on - strand at 2352.066 kb on - strand at 2352.951 kb on - strand at 2353.075 kb on - strand, within BWI76_RS11165 at 2353.102 kb on + strand, within BWI76_RS11165 at 2353.102 kb on + strand, within BWI76_RS11165 at 2353.110 kb on - strand, within BWI76_RS11165 at 2353.206 kb on + strand, within BWI76_RS11165 at 2353.214 kb on - strand, within BWI76_RS11165 at 2353.799 kb on - strand at 2353.821 kb on - strand at 2354.214 kb on - strand, within BWI76_RS11170 at 2354.721 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB29 MOI 1 remove 2,351,881 - BWI76_RS11155 0.78 +2.5 2,352,007 - +0.3 2,352,066 - +0.5 2,352,951 - +4.2 2,353,075 - BWI76_RS11165 0.23 +0.5 2,353,102 + BWI76_RS11165 0.26 +1.2 2,353,102 + BWI76_RS11165 0.26 +0.5 2,353,110 - BWI76_RS11165 0.27 -0.8 2,353,206 + BWI76_RS11165 0.36 +0.2 2,353,214 - BWI76_RS11165 0.37 +2.2 2,353,799 - -0.8 2,353,821 - +0.1 2,354,214 - BWI76_RS11170 0.59 +2.7 2,354,721 + +1.5
Or see this region's nucleotide sequence