Experiment: KLEB29 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10780 and BWI76_RS10785 are separated by 286 nucleotides BWI76_RS10785 and BWI76_RS10790 overlap by 4 nucleotides BWI76_RS10790 and BWI76_RS10795 are separated by 193 nucleotides
BWI76_RS10780: BWI76_RS10780 - pyrimidine utilization protein A, at 2,274,838 to 2,275,929
_RS10780
BWI76_RS10785: BWI76_RS10785 - transcriptional regulator, at 2,276,216 to 2,276,854
_RS10785
BWI76_RS10790: BWI76_RS10790 - hypothetical protein, at 2,276,851 to 2,277,246
_RS10790
BWI76_RS10795: BWI76_RS10795 - L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88); Proline dehydrogenase (EC 1.5.5.2) (from data) , at 2,277,440 to 2,281,402
_RS10795
Position (kb)
2276
2277
2278 Strain fitness (log2 ratio)
-1
0
1 at 2275.861 kb on + strand at 2275.864 kb on - strand at 2275.869 kb on - strand at 2275.984 kb on - strand at 2276.219 kb on + strand at 2276.219 kb on + strand at 2276.227 kb on - strand at 2276.227 kb on - strand at 2276.385 kb on + strand, within BWI76_RS10785 at 2276.393 kb on - strand, within BWI76_RS10785 at 2276.402 kb on - strand, within BWI76_RS10785 at 2276.482 kb on - strand, within BWI76_RS10785 at 2276.525 kb on - strand, within BWI76_RS10785 at 2276.525 kb on - strand, within BWI76_RS10785 at 2276.728 kb on + strand, within BWI76_RS10785 at 2276.728 kb on + strand, within BWI76_RS10785 at 2276.942 kb on + strand, within BWI76_RS10790 at 2277.019 kb on + strand, within BWI76_RS10790 at 2277.082 kb on + strand, within BWI76_RS10790 at 2277.125 kb on + strand, within BWI76_RS10790 at 2277.133 kb on - strand, within BWI76_RS10790 at 2277.303 kb on + strand at 2277.330 kb on - strand at 2277.515 kb on - strand at 2277.846 kb on - strand, within BWI76_RS10795 at 2277.961 kb on + strand, within BWI76_RS10795 at 2278.182 kb on + strand, within BWI76_RS10795
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB29 MOI 1 remove 2,275,861 + +0.6 2,275,864 - -0.6 2,275,869 - -0.9 2,275,984 - -0.4 2,276,219 + -0.4 2,276,219 + +0.3 2,276,227 - +0.7 2,276,227 - -0.7 2,276,385 + BWI76_RS10785 0.26 -0.0 2,276,393 - BWI76_RS10785 0.28 -0.1 2,276,402 - BWI76_RS10785 0.29 +0.7 2,276,482 - BWI76_RS10785 0.42 -0.1 2,276,525 - BWI76_RS10785 0.48 +0.0 2,276,525 - BWI76_RS10785 0.48 -1.4 2,276,728 + BWI76_RS10785 0.80 +0.2 2,276,728 + BWI76_RS10785 0.80 -0.1 2,276,942 + BWI76_RS10790 0.23 -1.5 2,277,019 + BWI76_RS10790 0.42 -0.4 2,277,082 + BWI76_RS10790 0.58 -1.3 2,277,125 + BWI76_RS10790 0.69 -0.4 2,277,133 - BWI76_RS10790 0.71 -0.4 2,277,303 + +0.2 2,277,330 - -0.3 2,277,515 - +1.3 2,277,846 - BWI76_RS10795 0.10 -1.1 2,277,961 + BWI76_RS10795 0.13 +1.6 2,278,182 + BWI76_RS10795 0.19 -0.4
Or see this region's nucleotide sequence